比较两种方法检测人乳头状病毒的敏感性和特异性  

Compare the Sensitivity and Specificity of the Method for Detection of Human Papillomavirus

作　　者：林虹[1] 张杰[1] 彭正科[1] 黄建宏[1] 

机构地区：[1]广东省珠海市人民医院检验科,广东珠海519000

出　　处：《中国医药指南》2012年第20期35-36,共2页Guide of China Medicine

摘　　要：目的比较两种方法检测人乳头状病毒(HPV)的敏感性和特异性。方法收集160例无妇科临床症状的女性,采用液基细胞学和荧光定量PCR法检测HPV感染,同时采用细胞病理学作为诊断金标准,比较两组检测方法的敏感性、特异性、费用和时间。结果①与液基细胞学相比,荧光定量PCR法具有较高的敏感性和特异性,差别具有统计学意义;②荧光定量PCR法检测时间长于液基细胞学(P=0.036),虽然荧光定量PCR法费用高于液基细胞学,但差别无统计学意义(P=0.058)。结论对于HPV的早期筛查,与液基细胞学相比,采用荧光定量PCR法具有较高的敏感性和特异性,值得临床推广。Objective To compare the sensitivity and specificity of two methods on the detection of HPV.Methods 160 women without other clinical symptoms were enrolled and liquid based cytology test （LCT） and real time PCR （RT-PCR） were used to detect HPV infection, and cytological pathology was used as golden standard. The sensitivity, specificity, cost and detected time were compared of these two methods. Results （1）Compared to the LCT, the sensitivity and specificity were significantly higher with RT-PCR; （2）and the detected time was longer with RT-PCR than with LCT（P=-0.036）, although the cost was higher with RT-PCR than with LCT, there was no statistically significant （P=0.058）. Conclusion Compared to the LCT, the sensitivity and specificity of early detection of HPV was higher with RT-PCR which deserved broadly application.

关 键 词：人乳头状病毒 液基细胞学 荧光定量PCR 

分 类 号：R737.33[医药卫生—肿瘤]