荧光PCR和蛋白免疫印迹技术在诊断新生儿先天梅毒中的意义  

Fluorescent PCR and Western blot technique in the diagnosis of neonatal congenital syphilis

作　　者：杨梅 

机构地区：[1]成都都江堰人民医院检验科,成都611830

出　　处：《中国性科学》2012年第8期42-44,共3页Chinese Journal of Human Sexuality

摘　　要：目的:探讨荧光PCR和蛋白免疫印迹技术在诊断新生儿先天梅毒中的运用。方法:对我院收治的50例先天梅毒患儿以及40例正常新生儿进行荧光PCR和蛋白免疫印迹技术在诊断新生儿先天梅毒的敏感度以及特异性研究。结果:荧光PCR其敏感度为98%,特异性为100%,明显高于蛋白免疫印迹的84%以及85%。两种方法比较具有统计学差异性(P<0.05)。结论:荧光PCR在诊断新生儿先天梅毒中较蛋白免疫印迹技术具有更好的敏感性以及特异性,值得在临床中推广。Objectives： To investigate the effect of fluorescent PCR and Western blot technique in the diagnosis of neonatal congenital syphilis. Methods： Fifty cases of children with congenital syphilis and 40 cases of normal newborns were selected to be applied with the fluorescent PCR and WeStern blot technique. We studied the specificity and sensitivity of these techniques in the diagnosis of neonatal congenital syphilis. Results： The sensitivity and specificity of Fluorescent PCR were 98% and 100%, sig- nificantly higher than that of Western blot （ 84% and 85 % respectively） （P 〈 0. 05）. Conclusions： Fluorescent PCR has better sensitivity and specificity than the Western blot technique in the diagnosis of neonatal congenital syphilis

关 键 词：荧光PCR 蛋白印迹 先天梅毒 

分 类 号：R737.33[医药卫生—肿瘤]