大肠癌患者外周血CK-20mRNA基因表达的初步研究  

Preliminary study of CK-20mRNA gene expression in peripheral blood of patients with colorectal carcinoma

作　　者：叶欣[1] 李毅[1] 刘忠民[1] 陈廷武 张军[1] 杨宪勇[1] 

机构地区：[1]泰山医学院第一教学医院,山东泰安271000

出　　处：《泰山医学院学报》2000年第2期134-136,共3页Journal of Taishan Medical College

基　　金：山东省医药卫生科研基金!(1999.第A6号 )

摘　　要：目的　探讨大肠癌患者外周血CK 2 0mRNA(细胞角蛋白 2 0mRNA)基因表达及其临床意义。方法　用RT PCR(逆转录 多聚酶链反应 )法检测了 34例大肠癌患者外周血CK 2 0mRNA的表达情况。结果　按Dukes分期外周血CK 2 0mRNA的表达率分别是 :A期 2 0 % ,B期 37 5 % ,C期 5 3 3 % ,D期 83 3 %。结论　大肠癌患者外周血CK 2 0mRNA的基因表达与Dukes分期有一定关系 。Objective:To explore CK 20mRNA expression and its clinical significance in peripheral blood of patients with colorectal carcinoma.Methods: The expression of CK 20mRNA was detected by RT PCR in 34 cases.Results: According to Dukes stage,the positive rates of CK 20mRNA in peripheral blood were 20%(A),37.5%(B),53.3%(C), 83. 3%( D) , respectively.Conclusion:The expression of CK 20mRNA in peripheral blood is related with Dukes stage and is an indicator of blood micrometastasis of colorectal carcinoma.

关 键 词：大肠癌 CK-20mRNA 外周血 RT-PCR 

分 类 号：R735.34[医药卫生—肿瘤]