呼吸道感染患者奈瑟菌属致病性相关基因的检测  

Detection of pathogenic genes in Neisseria sppcausing respiratory tract infections

作　　者：罗振华[1] 王和[1] 易旭[1] 佘晓玲[1] 王涛[1] 王艳[2] 叶长芸[2] 

机构地区：[1]贵阳医学院微生物学教研室,贵州贵阳550004 [2]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中华医院感染学杂志》2012年第15期3216-3218,共3页Chinese Journal of Nosocomiology

基　　金：贵州省科技基金(SY[2001]3045);贵州省卫生厅基金(gzwkj2009-1-028);传染病预防控制国家重点实验室自主研究开放课题基金(2011SKLID302)

摘　　要：目的检测呼吸道感染患者分离奈瑟菌属的致病性相关基因,探讨非淋病奈瑟菌、非脑膜炎奈瑟菌的致病性。方法采用PCR扩增和序列分析技术,对慢性呼吸道感染患者下呼吸道分离奈瑟菌属致病性相关基因orf1与nspA进行检测。结果从呼吸道感染患者标本分离的230株奈瑟菌属,orf1基因阳性35株,序列比对与淋病奈瑟菌TCDC-NG08107 orf1序列同源性达95.0%～99.0%;nspA无阳性反应。结论人体上呼吸道正常菌群奈瑟菌属缺乏nspA基因,少数菌株可具有淋病奈瑟菌及脑膜炎奈瑟菌致病性相关基因orf1。OBJECTIVE To detect the pathogenic genes of Neisseria spp. isolated from patients with respiratory tract infections and probe the pathogenicity of non-gonococci and non-meningitidis Neisseria species. METHODS The virulence-associated genes orfl and nspA in Neisseria species isolated from the patients with chronic respiratory tract infections were detected and analyzed by PCR and nucleotide sequencing. RESULTS Of the 230 strains of Neisseria species isolated from patients with chronic respiratory tract infections, totally 35 strains showed orfl positive, as compared with the sequence of TCDC-NG08107 orfl of N. gonorrhoeae, the sequence homology reaching to 95.0^--99.0%, and there was no positive reaction of nspA. CONCLUSION The species of Neisseria spp. which are the normal flora of upper respiratory tract of human lack nspA, the pathogenic gene off1 of N. gonorrhoeae and N. meningitides can be found only in few strains, which indicate that the genes are not the important virulence factors for the normal flora Neisseria species living the upper respiratory tract of human that causing the secondary respiratory tract infections.

关 键 词：奈瑟菌属 淋病 致病基因 诊断 

分 类 号：R378[医药卫生—病原生物学]