双色荧光原位杂交检测放射性工作者的染色体畸变及剂量估算  

Detection of Chromosome Aberration by Dual Color Fluorescence in situ Hybridization and Exposure Assessment of Radiation Exposed Workers

作　　者：徐永忠[1] 李亘山 郑斯英[1] 赵经涌[1] 朱巍[1] 曹建平[1] 

机构地区：[1]苏州大学放射医学系,苏州市215007 [2]南京市卫生防疫站

出　　处：《工业卫生与职业病》2000年第4期196-198,共3页Industrial Health and Occupational Diseases

基　　金：国防科工委基金!资助(Y55732 62 )

摘　　要：应用荧光原位杂交技术检测 13例放射性工作者的外周血淋巴细胞染色体易位率及双着丝粒体率 ,并根据易位率进行生物剂量估算。结果表明 ,13例放射性工作者的平均易位率、双着丝粒体率都显著高于对照组 (P <0 0 1) ,13例放射性工作者的平均易位率约为双着丝粒体率的 8 44倍 ,两者差异显著(P <0 0 1) ,且两者无相关性 (r =0 44 3) ,除 1例核医学工作者外 ,其余 12例的生物剂量估算范围在0 6 2～ 2 17Gy之间。提示 。Translocations and dicentrics in peripheral blood lymphocytes of 13 radiation exposed workers were observed by fluorescence in situ hybridization(FISH)and their biological doses were estimated based on translocation frequencies.The mean translocation frequency and dicentric frequency of all 13 radiation exposed workers were significantly elevated compared with the control group(P<0 01).For a group of 13 radiation workers,the mean translocation frequency was significantly higher than the mean dicentric frequency(P<0 01),and no correlation was observed between them(r=0 443).Except a nuclear medical worker,the individual dose ranged 0 62 and 2 17Gy.The results indicated that translocation detected by FISH could be applied to estimate the biological doses of radiation workers.

关 键 词：荧光原位杂交 剂量 染色体畸变 放射性工作者 

分 类 号：R144[医药卫生—公共卫生与预防医学] X591[环境科学与工程—环境工程]