卵巢癌多药耐药相关药物的体外敏感性与MDR1基因表达  

In Vitro Chemosensitivity of Ovarian Cancer to MDR Related Drugs and Expression of MDR1 Gene

作　　者：刘东光[1] 

机构地区：[1]山东济宁市第一人民医院,山东济宁272111

出　　处：《实用妇产科杂志》2000年第4期196-197,共2页Journal of Practical Obstetrics and Gynecology

摘　　要：目的 :探讨卵巢癌多药耐药 (MDR)相关药物的体外敏感性与 MDR1基因表达的关系 ,为卵巢癌合理化疗提供理论依据。方法 :采用四甲基偶氮唑蓝 (MTT)比色法体外药物敏感 (药敏 )试验及逆转录—聚合酶链反应 (RT- PCR)技术 ,检测 37例卵巢癌对 MDR相关药物的敏感性 ,及其 MDR1基因表达情况。结果 :卵巢癌 MDR1基因表达阳性率为30 % ,与 MDR相关药物耐药有关 ,与非 MDR相关药物耐药无关。结论 :MDR1基因表达是卵巢癌抗 MDR相关药物的主要原因。Objective:To study the relationship between in vitro chemosensitivity of ovarian cancer (OC) to MDR related drugs and expression of MDR1 gene.Methods:Sensitivity of OC to clinical common anticancer agents was examined by means of MTT assay in vitro.Reverse transcription-polymerase chain reaction(RT-PCR)assay was used for dection of MDR1 gene expression in 37 OC.Results:MDR1 gene expression was detected in 30% of OC analysed.The level of MDR1 mRNA was not correlated with non MDR related drugs,but correlated with MDR related drugs.Condusion:These results support the idea that the increased expression of MDR1 gene is a major cause of resistance to MDR related drugs.

关 键 词：卵巢肿瘤 多药抗药性 基因表达 聚合酶链反应 

分 类 号：R737.31[医药卫生—肿瘤]