高效液相法测定叶酸片中叶酸的含量  

Determination of Folic Acid in Folic Acid Tablet by High Performance Liquid Chromatography

作　　者：陈航[1] 邓丽明[2] 

机构地区：[1]江苏省食品药品检验所,江苏南京210008 [2]东南大学附属中大医院,江苏南京210009

出　　处：《分析科学学报》2012年第4期587-589,共3页Journal of Analytical Science

摘　　要：建立了高效液相色谱法测定叶酸片中叶酸含量的方法。采用Symmetry C18色谱柱(150×4.6mm,3.5μm),以磷酸盐缓冲溶液(PBS,pH=6.3)为流动相,检测波长254nm,柱温35℃。叶酸在0.04～0.36μg/mL范围线性良好(r=0.9999),平均加标回收率为99.4%(RSD=0.44%)。该方法简便准确,精密度良好,适用于叶酸片中叶酸含量的测定。An HPLC method for the assay of folic acid in folic acid table was established. The assay was performed on a Symmetry C^8 column（150 X 4.6 mm, 3.5 ffm）. The mobile phase was phosphate buffer solution（pH=6.3）. The detection wavelength was 254nm and the column temperature was 35 ~C. Good linearity was obtained in the range of 0.04~0.36 g/mL（r=0. 9999）. The average recovery was 99.4% （RSD=0.44%）. The method was simple,rapid,accurate and might be suitable for the analysis of fo acid.

关 键 词：高效液相色谱法 叶酸 内标法 

分 类 号：O657.72[理学—分析化学]