人脑胶质瘤细胞白细胞介素-6的基因表达及自分泌环路的验证  

The expression of IL-6 gene in human glioma cells and the certification of IL-6 autocrine circle

作　　者：李刚[1] 李新钢[1] 张庆林[1] 田志刚 江玉泉[1] 张建华 

机构地区：[1]山东医科大学附属医院神经外科,济南250012 [2]山东省医科院基础所

出　　处：《中华神经外科杂志》2000年第4期232-234,共3页Chinese Journal of Neurosurgery

摘　　要：目的　验证新鲜人脑胶质瘤细胞是否能自发分泌和表达白细胞介素 6 (interleukin 6 ,IL 6 )并进一步明确IL 6在人脑胶质瘤发生及发展中的作用。方法　采用手术中直接获得的人脑胶质瘤组织 ,进行胶质瘤细胞的原代培养 ,对瘤细胞培养上清进行IL 6生物活性检测 ,然后采用逆转录聚合酶链反应 (reversetranscription polymerasechainreaction ,RT PCR)方法测定胶质瘤细胞中IL 6mRNA的表达 ;在确定人脑胶质瘤细胞有IL 6的分泌及表达后 ,再分别采用重组IL 6 (recombinantIL 6 ,rIL 6 )及IL 6单克隆抗体 (monoclonalantibody ,McAb)处理脑胶质瘤细胞。结果　原代培养的人脑胶质瘤细胞在无任何刺激的情况下可自发分泌IL 6 ,并在一定范围内呈剂量依赖性关系 ,而正常脑胶质细胞则无IL 6的分泌。 2 0例人脑胶质瘤标本中有 17例表达IL 6mRNA ,且其表达量与脑胶质瘤的恶性程度呈正相关关系。rIL 6能刺激脑胶质瘤细胞的增殖 ,而IL 6McAb则能抑制脑胶质瘤细胞的增殖。结论　新鲜人脑胶质瘤细胞亦能自发分泌和表达IL 6 ,人脑胶质瘤中也存在IL 6的自分泌环路 。Objective To study the secretion and expression of IL 6 in isolated fresh glioma cells, and to make sure the exact action of IL 6 in the occurring and developing of human gliomas.Methods Culturing the fresh isolated human glioma cells at different time, then assaying the biological activity of IL 6 in the supernatant of glioma cells. Using the RT PCR method to detect the expression of IL 6 mRNA in human glioma cells. Using different concentrations of rIL 6 and IL 6 McAb to act on fresh isolated human glioma cells, to observe the different functions of them to the proliferation of glioma cells.Results The isolated fresh human glioma cells can secrete IL 6 spontaneously without any stimulants in a dosedependent way, and its secretion can be promoted slightly by LPS. IL 6 McAb can neutralize the biological activity of IL 6 in the supernatant of human glioma cells. The expression of IL 6 mRNA was detectable in 17 tumor specimens(85%), which higher malignant tumors expressed significantly larger amount of IL 6 mRNA compared to lower grade tumors. rIL 6 can stimulate the proliferation of human glioma cells while IL 6 McAb can suppress it in a dose dependent way.Conclusions The isolated fresh human glioma cells can secrete and express IL 6 spontaneously. The IL 6 autocrine circle in the human gliomas also exist, and this circle may play an important role in sustaining the malignant proliferation of human gliomas.

关 键 词：脑胶质瘤细胞 白细胞介素6 基因表达 自分泌环路 

分 类 号：R739.41[医药卫生—肿瘤]