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作 者:陈红梅[1,2] 李金花[1,2] 柴兆祥[1] 郭成[3] 王蒂[2]
机构地区:[1]甘肃农业大学草业学院,兰州730070 [2]甘肃省作物遗传改良与种质创新重点实验室,兰州730070 [3]甘肃省农业科学院植物保护研究所,兰州730070
出 处:《植物保护学报》2012年第4期308-314,共7页Journal of Plant Protection
基 金:国家科技支撑计划(2012BAD06B03);甘肃省科技重大专项(1102NKDA025);甘肃省教育厅研究生导师科研资助项目(1002-05);现代农业产业技术体系(CARS-10-P18)
摘 要:为合理利用抗源,用菌株混合接种法分别接种接骨木镰刀菌Fusarium sambucinum和茄病镰刀菌F.solani,以DPS2000数据处理系统对接种块茎第15天腐烂斑的半径进行系统聚类分析,从而探讨35个马铃薯品种对镰刀菌干腐病优势病原的抗病性。结果表明:以接种接骨木镰刀菌的块茎其腐烂半径原始数值不转换、接种茄病镰刀菌的块茎其腐烂半径原始数值进行标准化转换、聚类方法均采用欧式距离、最长距离法,其聚类效果与接种结果的吻合度最好。接种接骨木镰刀菌后,以欧式距离21作为最佳聚类距离分割点,将试验品种分为11个抗病品种、17个中抗品种和7个感病品种;接种茄病镰刀菌后,以欧式距离3.0作为最佳聚类距离分割点,将试验品种分为13个抗病品种、14个中抗品种和8个感病品种。In order to reasonably utilize resistant potato resources and cost-effectively control potato Fu- sarium dry rot, resistance of 35 potato varieties to the dominant pathogens, F. sambucinum and F. solani, were evaluated. Tubers of these varieties were inoculated with the mixture of F. sambucinum isolates and the mixture of F. solani isolates respectively. The radius of rotting spots of inoculated tubers on the 15th day after inoculation was analyzed via DPS2000 Data Processing System. The results showed that the best clustering effect were at the optimal composition of the original data unconverted for Fusarium sambuci- num, and the original data standardized for F. solani, combined with euclidean distance and the longest distance method together, which had best degree of agreement with the inoculation results. The 35 potato varieties were grouped into three groups of 11 resistant, 17 medium resistant and 7 susceptible varieties at grouping point 21 of euclidean distance for tubers' resistance to F. sambucinum, resistant, 14 medium resistant and 8 susceptible varieties at grouping point 3.0 while three groups of 13 of euclidean distance for resistance to F. solani.
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