高分辨率熔解曲线检测大肠癌KRAS基因突变  

High resolution melting analysis of KRAS mutation in colorectal cancer

作　　者：张彩凤[1] 夏永华[2] 李贞娟[1] 韩宇[1] 

机构地区：[1]新乡医学院第一附属医院消化内科,河南卫辉453100 [2]新乡医学院第一附属医院皮肤科,河南卫辉453100

出　　处：《中国现代医学杂志》2012年第21期29-33,共5页China Journal of Modern Medicine

基　　金：河南省教育厅科研项目(No:20073200143)

摘　　要：目的研究高分辨率熔解曲线(HR M)检测大肠癌KR AS突变的准确性及敏感性。方法采用HR M检测大肠癌KR AS基因外显子2和3突变情况并与直接测序比较,研究HR M准确性。用KR AS野生型样本系列稀释KR AS外显子2第12个密码子突变型(0,1%,5%,10%,15%),采用HR M检测,评价其灵敏度。结果81例大肠癌肿瘤组织石蜡标本,检测到11例KR AS外显子2突变(13.58%),外显子3无突变。162例正常组织标本未检测到突变。HRM检测与测序结果一致率100%。HRM可检测到1%KRAS外显子2第12个密码子突变型。结论 HRM可以准确定性的检测KRAS基因突变分型,灵敏度达1%。To study accuracy and sensitivity of High Resolution Melting（HRM） of KRAS（v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog） mutation in colorectal cancer.【Methods】 HRM was used to detect exon 2 and 3 of KRAS mutation on DNA in the same sample of colorectal cancer,the results were compared with direct sequencing data.Serially dilute the KRAS exon 2 mutation samples with KRAS WT samples（0,1%,5%,10%,and 15%）;the sensitivity of HRM was analyzed by detecting the samples.【Results】 Overall,81 cases of paraffin-embedded tumor tissues from patients with colorectal cancer were detected,and we found KRAS mutations of 11 cases on exon 2（13.58%） and no case on exon 3.No case was detected any mutation on exon 2 and 3 in normal tissues.The accuracy of HRM is 100%,and the sensitivity of HRM is 1%.【Conclusion】 HRM can accurately distinguish between KRAS wild type and mutant,and the sensitivity can be up to 1%.

关 键 词：高分辨率熔解法 KRAS基因 大肠癌 准确性 灵敏度 

分 类 号：R735.34[医药卫生—肿瘤]