p38MAPK通路介导胰高血糖素样肽-1对人脐静脉内皮细胞凋亡的抑制  

Involvement of p38 MAPK pathway in GLP-1-induced inhibition of apoptosis in human umbilical vein endothelial cells

作　　者：徐华[1] 李海龙 牛自勇[2] 李桂忠[1] 曹军[1] 姜怡邓[1] 

机构地区：[1]宁夏医科大学基础医学院病理生理学系,银川750004 [2]宁夏第三人民医院内科,银川750021

出　　处：《生理学报》2012年第4期444-448,共5页Acta Physiologica Sinica

基　　金：supported by Key Scientific Research Project of Health Department of Ningxia Hui Autonomous Region (No.2012118);the National Natural Science Foundation of China (No. 30960124,81160044)

摘　　要：本文旨在探讨胰高血糖素样肽-1 (glucagon-like peptide-1, GLP-1)对棕榈酸盐诱导的人脐静脉内皮细胞(human umbili-cal vein endothelial cells, HUVECs)凋亡的影响及其机制。体外培养HUVECs,用棕榈酸盐诱导其凋亡,同时加入GLP-1干预。酶联接免疫吸附剂测定(enzyme-linked immunosorbnent assay, ELISA)法分别测定Caspase-3活性和DNA碎片含量,Westernblot测定磷酸化细胞丝裂原活化蛋白激酶(phospho-p38 mitogen-activated protein kinase, p-p38 MAPK)表达。结果显示,与对照组相比,棕榈酸盐处理的HUVECs中Caspase-3活性和DNA碎片含量显著增多;而GLP-1可呈剂量依赖性降低棕榈酸盐所诱导升高的Caspase-3活性和DNA碎片。另外,GLP-1显著抑制由棕榈酸盐诱导的HUVECsp-p38 MAPK表达上调。以上结果提示,GLP-1可以抑制棕榈酸盐诱发的HUVECs脂性凋亡,其机制可能是对p-p38 MAPK表达的下调。The aim of the present study was to investigate the effect of glucagon-like peptide-1 （GLP-1） on palmitate-induced apoptosis of human umbilical vein endothelial cells （HUVECs） and the underlying mechanism. HUVECs were cultured in vitro, and then treated by palmitate to induce apoptosis. Meanwhile, GLP-1 was added to explore its effect. After 24 h of the treatments, Caspase-3 activity and DNA fragmentation were measured using ELISA kits. Phospho-p38 mitogen-activated protein kinase （p-p38 MAPK） expression was detected by Western blot. The results showed that incubating HUVECs with 0.125 mmol/L GLP-l increased Caspase-3 activity and DNA fragmentation. GLP-1 significantly inhibited palmitate-induced increases of Caspase-3 activity and DNA fragmentation in a concentration-dependent manner. Moreover, GLP-1 inhibited the up-regulation of p-p38 MAPK expression induced by palmitate in HUVECs. These results suggest GLP-1 protects HUVECs against lipo-apoptosis, and this effect may be mediated through inhibiting p38 MAPK pathway.

关 键 词：胰高血糖素样肽-1 人脐静脉内皮细胞 P38 MAPK 凋亡 

分 类 号：R363.2[医药卫生—病理学]