一株具有抑菌活性的粉背雷公藤内生真菌的分离鉴定  

Isolation and identification of an antibacterial endophytic fungus from Tripterygium hypoglaucum(Lev1.)Hutch.

作　　者：刘云[1] 朱欣婷[2] 李常春[3] 郭阁[2] 方玉荣[2] 廖维[2] 

机构地区：[1]遵义医学院医学与生物学研究中心,贵州遵义563003 [2]遵义医学院基础医学院,贵州遵义563003 [3]遵义医学院药学院,贵州遵义563003

出　　处：《广东农业科学》2012年第15期76-78,共3页Guangdong Agricultural Sciences

基　　金：贵州省科技厅;遵义医学院;遵义市科技局联合资金项目(黔科合J字LKZ[2010]48号);遵义市红花岗区科学技术项目(遵红科合社字[2009]15号)

摘　　要：从粉背雷公藤新鲜植株中分离筛选具有抑菌活性的内生真菌。采用组织块培养法分离得到107株内生真菌。以金黄色葡萄球菌、大肠埃希杆菌、变形球菌、枯草芽孢杆菌作为指示菌进行体外抑菌试验,通过滤纸片扩散法筛选出一株具有研究潜力的抑菌活性菌株(ETH-1),菌株ITS序列的系统发育分析结果表明ETH-1为拟茎点霉属真菌。Isolation and screening of an antibacterial endophytic fungus from Tripterygium hypoglaucum （Levi.）Hutch..0ne hundred and seven endophytic fungus were isolated by issue-culture method. Four bacteria, Staphylococcus oureus, Escherichia coli, Deformation coccus and Bacillus subtilis, were used as indicators for antibacterial experiment in vitro. An antibacterial endophytic fungus named ETH-1 which was identified as Phomopsis sp. based on phylogenetic analysis of ITS sequences was screened by filter paper dispersion.

关 键 词：粉背雷公藤 内生真菌 抑菌 

分 类 号：S482.292[农业科学—农药学]