ERβ在骨缺损模型愈合中的作用  

Role of ERβ in the Healing of Bone Defect Model

作　　者：胡新佳[1] 黄智敏[1] 陈启明[1] 潘晓华[1] 李刚[2] 

机构地区：[1]暨南大学附属第二医院,深圳市人民医院骨关节科,广东深圳518020 [2]香港中文大学医学院矫形外科及创伤学系

出　　处：《医学临床研究》2012年第7期1217-1219,共3页Journal of Clinical Research

基　　金：广东省深圳市2011年度科技计划项目（编号201102143）

摘　　要：【目的】探讨雌激素受体β（ERβ）在骨修复中的作用。【方法】制作雌性C57小鼠（对照组）与ERp基因敲除（ERβ^-/-）小鼠（实验组）两组小鼠单侧股骨滑车（d=0．6mm）的全层软骨下骨缺损模型，于术后1、2、3、4周取材，应用MircoCT扫描，比较两组在缺损模型愈合过程中的骨体积分数（BV／TV）的差异来对比观察骨修复过程。【结果】两组骨缺损模型在1周内即开始出现新骨，4周已骨性愈合。实验组与对照组骨缺损区域BV／TV值随时间变化的趋势不同；实验组在1周、3周和4周三个时间点BV／TV值均高于对照组，且差异有显著性（P〈0．05）。BV／TV值时间效应与处理交互效应也有统计学意义（F=2259．412，F=15．207，均P=0．000）。【结论】ERβ在骨修复过程中呈现负性调节新骨形成的作用。[Objective]To explore the role of estrogen receptor-beta（ERβ） in bone repair. [Methods]Fullthickness subchondral bone defect models of unilateral trochlea（d= 0.6mm） of femur of female C57 mice（con- trol group） and ERβ^-/- mice（experimental group） were established. After 1, 2, 3 and 4 weeks of operation, the samples were collected. Mirco CT scan was performed. The process of bone repair was observed by com- paring bone volume fraction（BV/TV） in the process of bone healing of defect model in two groups. [Results] New bone formation was found in bone defect models of two groups Within a week. Bone union was completed at 4 weeks. The time-dependent change of BV/TV in bone defect region of experimental group was different from that of control group. BV/TV in experimental group at 1-, 3- and 4-week time points was higher than that in control group, and there was significant difference（ P 〈0.05）. Time and treatment interaction of BV/ TV also had statistical significance（F=2259. 412, F=15. 207, all P =0. 000）. [Conclusion] ERβ plays a role in down-regulation of new bone formation in the process of new bone formation.

关 键 词：骨疾病 疾病模型 动物 

分 类 号：R681[医药卫生—骨科学]