枯否细胞在昆明小鼠实验性肝癌发生中的作用及机制  

Study on the Role of Kupffer Cells in the Carcinogenesis of Experimental Liver Carcinoma of Kunming Mouse

作　　者：王梦远 李旭宏 何天时 苟剑林 

机构地区：[1]重庆市万州区三峡中心医院普外二科,重庆404000

出　　处：《医学临床研究》2012年第7期1261-1264,共4页Journal of Clinical Research

摘　　要：【目的】研究枯否细胞（KCs）与肝细胞癌（HCC）发生发展的相关性。【方法】60只昆明小鼠，分成三组：GdCl3预处理组（A组）；肝癌组（B组）；正常对照组（C组）。绘制Kaplan-Meier生存曲线比较各组生存期；光镜下观察肿瘤组织生长情况，免疫组织化学染色鉴定肝脏及肿瘤组织KCs并观察其分布范围和及计数。【结果】①生存曲线显示：A，B组生存时间逐渐缩短，且差异具有显著性（P〈0．05）。②H22肿瘤细胞接种3周后，肉眼观察A组癌灶的体积明显大于B组，差异具有显著性（P〈0．05）。③HE染色显示光镜下可见三组病理组织学变化差异显著。④A组、B组的癌旁肝组织及远癌肝组织的KCs数量较C组明显增加（P〈0．01），但两组之间差异无显著性（P〉0．05）。⑤A、B及C组各组小鼠血清TNF-α水平有显著性差异（P〈0．01）；【结论】KCs有保护肝细胞、抑制其恶性转化的功能。[Objective]To study the relationship between Kupffer cells（KCs） and the genesis and development of hepatic cell carcinoma（HCC）. [Methods]Sixty Kunming mice were randomly divided into gadolinium ehioride（Gdcl3） pretreatment group（group A）, HCC group （group B） and normal control group（group C）. Survival curve was drawn for comparing the survival time at difference stages. Tumor growth was observed under light microscope. Immunohis- tostaining method was used to identify KCs in liver and tumor tissues and observe the distribution and count. [Results] Kaplan-Meier survival curve showed that survival time of group A and group B was decreased gradually, and there was significant difference（ P 〈0.05）. After 3 weeks of tumor cell inoculation, macroscopic observation showed that cancer lesion volume in group A was bigger than that in group B, and there was significant difference（ P 〈0.05）. HE staining showed that there was significant difference in pathohistology among 3 groups under light mi- croscope. Compared with group C, the number of KCs in adjacent liver tissue and farther liver tissue of group A and group B increased（ P 〈0.01）, but there was no significant difference between group A and group B（ P 〉0.05）. There was significant difference in serum TNF-α level in mice of group A, B and C（ P 〈0.01）. [Conclusion] KCs has the effect for protecting liver cell and inhibit its malignant transformation.

关 键 词：肝肿瘤 实验性 枯否氏细胞 小鼠 

分 类 号：R575.2[医药卫生—消化系统]