HP-β-CD对脂质体和真核细胞通透性的影响  

Influence of HP-β-CD on the Penetration of Nuclear Microbial Cell and Liposome

作　　者：崔兰玉 王敏[1] 申雁冰[1] 骆健美[1] 郑宇[1] 

机构地区：[1]工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2012年第4期7-11,共5页Journal of Tianjin University of Science & Technology

基　　金：国家自然科学基金资助项目(21076158);教育部新世纪优秀人才计划(NCET-08-0911)

摘　　要：以薄膜水化法制备的阴离子脂质体的平均粒径为(180±2.3)nm,Zeta电位为–33.9,mV.分别以脂质体中钙黄绿素的泄露量和紫外分光光度法测定蛋白含量,以表征羟丙基-β-环糊精(HP-β-CD)对脂质体和真核微生物蓝色犁头霉(Absidia coerulea)细胞通透性的影响.结果表明:在HP-β-CD作用下,两者的通透性变化趋势一致.分析比较HP-β-CD作用下的甾体化合物17,а,21-二羟基孕甾-4-烯-3,20-二酮(RS)在脂质体和菌体细胞中的通量变化的相关性,表明脂质体与细胞通量变化的拟合度达到97.9%,相关性显著(P<0.05).由此说明,本研究建立的脂质体制备方法以及获得的脂质体能够模拟真核细胞,应用于真核微生物的生物学研究.Liposome with negative charge was prepared by film hydration method. The average diameter of the liposome was about 180- 2.3 nm and zeta potential was about -33.9 mV. The effect of hydroxypropyl-β-cyclodextrin（HP-β- CD） on the penetration of liposome was determined by calculating the leakage of calcium. With the increase of the concentra- tion of HP-β-CD and cholesterol, the penetration of liposome increased. Similarly, the penetration ofAbsidia coerulea cells increased when the concentration of HP-β-CD increased. The penetration of steroid 17 a-Hydroxypregna-4-ene-3,20- dione （RS）into liposome had preferable relationship with the results of RS having penetrated into Absidia coerulea cells, and the relative coefficient was 97.9%. The liposome could be used to investigate the penetration of steroid into cells.

关 键 词：脂质体 羟丙基-Β-环糊精 蓝色犁头霉 通透性 

分 类 号：Q31[生物学—遗传学]