弥漫性脑外伤对大鼠内耳超微结构及听功能影响的实验研究  

Experimental study of diffuse traumatic brain iljury on inner ear ultrastructure and auditory function ofrats

作　　者：付兴华[1] 邓艳丽[1] 张萍[1] 顾洪兰[1] 赵君晖[1] 郑学斌[2] 韩莉[1] 张再兴[1] 张文丽[3] 李倩[3] 卢鹤翔 孙瑞军 

机构地区：[1]河北联合大学附属医院耳鼻咽喉科,河北省唐山市063000 [2]唐山市公安局刑警支队法医化验大队 [3]河北联合大学实验中心电镜室 [4]形态中心

出　　处：《中国医药》2012年第9期1081-1084,共4页China Medicine

基　　金：河北省科学技术研究与发展计划（08276101D-74）

摘　　要：目的探讨弥漫性脑外伤对大鼠内耳超微结构及听功能的影响。方法建立弥漫性脑外伤大鼠模型并完全随机分成正常对照组及外伤1、2、3、4周组，每组各30例。采用听性脑干反应测定及光镜、电镜观察等手段，记录各波波峰潜伏期（PL）及各波峰间潜伏期（IPL），观察各组动物耳蜗超微结构及听功能的变化。结果正常对照组大鼠耳蜗结构正常，外伤后各组内耳超微结构有明显损伤变化。外伤1、2、3、4周组V波阈值均高于正常对照组[分别为（18．50±7．83）、（17．83±8．35）、（16．00±7．12）、（15．42±7．21）dB比（1．17±2．13）dB]，差异均有统计学意义（均P〈0．05）。同时外伤1、2、3、4周组I波、Ⅲ波、Ⅳ波PL及I-Ⅲ、Ⅲ-V、I-V IPL均明显高于正常对照组，差异均有统计学意义（P〈0．05）。结论弥漫性脑外伤对内耳超微结构及听功能均有不同程度的影响，听功能变化可能与耳蜗超微结构损伤有关。Objective To explore the effects of diffuse traumatic brain injury on inner ear ultrastructure and auditory function of rats. Methods Diffuse traumatic brain injury model of rats was established and all 150 rats were randomly divided into control group and experimental groups （including 1-, 2-, 3-, 4-week after injury group） （ n -- 30 in each group）. Then auditory brainstem response examination, light microscope and electron microscope technique were used to evaluate the change of hearing function and morphology. The peak latency （ PL）, the interpeak latency（IPL） were recorded and compared. Results The control group of rats cochlear structures was normal, whereas there were changes of inner ear ultrastructure in the traumatic groups. The threshold values in 1-, 2-, 3-, 4-week after injury group were higher than those in control group [ （ 18.50 ± 7.83 ）, （ 17. 83 ± 8. 35 ）, （16.00±7.12）, （15.42 ±7.21）dB vs（1.17 ±2.13）dB] （all P〈0.05）. Meanwhile, the PL and IPL in experimental groups were higher than those in control group （ all P 〈 0. 05 ）. Conclusions Diffuse traumatic brain injury can affect the inner ear ultrastrueture and auditory function of rats. The auditory function may be related with the ultrastructure change of inner ear.

关 键 词：弥漫性脑外伤 内耳 超微结构 听力 

分 类 号：R764[医药卫生—耳鼻咽喉科]