Ⅱ型糖尿病前期伴牙龈炎患儿牙周可疑致病菌的检测  

Detection of putative periodontal pathogens in the subgingival plaque of type Ⅱ prediabetes children with gingivitis

作　　者：蔡梁婧[1] 邰佰霞[1] 王永兰[1] 郭宏磊[1] 朱志颖[1] 

机构地区：[1]天津医科大学口腔医院,天津300070

出　　处：《牙体牙髓牙周病学杂志》2012年第8期458-462,共5页Chinese Journal of Conservative Dentistry

摘　　要：目的:检测Ⅱ型糖尿病前期伴牙龈炎患儿龈下菌斑中的牙周可疑致病菌,指导牙周病的治疗和预防。方法:以6～14岁的儿童作为研究对象,按照血糖水平和牙龈炎症状况分为3组:糖尿病前期伴牙龈炎组(实验组)、代谢正常伴牙龈炎组(对照组一)、代谢正常而牙周健康组(对照组二),每组各30例。以牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)、具核梭杆菌(Fusobacterium nucleatum,Fn),中间普氏菌(Prevotella intermedia,Pi),福塞坦氏菌(Tannerella forsythia,Tf)为目标菌,应用以16SrRNA为基础的聚合酶链反应(polynerase chain reaction,PCR)技术对龈下菌群进行检测。分析5种菌的相对含量与牙周临床指标和血糖水平的相关性。结果:5种可疑致病菌的检出率实验组明显高于对照组二(P<0.05);实验组虽高于对照组一,但无统计学差异(P>0.05)。Pg的相对含量与牙周探诊深度(Probing depth,PD)、龈沟出血指数(Sulcus bleeding index,SBI)以及空腹血糖(Fasting blood-glucose,FBG)存在相关性(P<0.05);Pi的相对含量与PD、SBI、FPG、口服葡萄糖耐量实验2 h血糖(OGTT2hBG)存在相关性(P<0.05);Aa和Fn的相对含量与SBI、PD存在相关性(P<0.05);Tf的相对含量与SBI、PD、FBG存在相关性(P<0.05)。结论:Ⅱ型糖尿病前期伴牙龈炎者牙周可疑致病菌的检出率明显高于正常对照组,虽与单纯牙龈炎组相比,其数量无明显差异,但应更加注意血糖水平和菌斑控制。AIM： To detect putative periodontal pathogens in the subgingival plaque of type II prediabetes children with gingivitis. METHODS： Six to 14 years old type II prediabetes children with gingivitis （experimental group）, children with only gingivitis （control） and healthy children （normal control） were included in this study, with 30 subjects in each group. Subgingival plaque was taken for the detection of Porphyromonasgingivalis （ Pg）, Actinoba- cillus actinomycetemcomitans （ Aa ） , Fusobacterium nucleatum （Fn）, Prevotella intermedia （Pi） and TannereUa for- sythia （Tf） by polyerase chain reaction （PCR）. The correlation of periodontal pathogens with clinical periodontal indi- ces was analyzed by regression analysis using SPSS software. RESULTS ： The detection rates of Pg, Aa, Fn, Tf and Pi were significantly higher in the experimental group than in the normal control group （ P 〈 0.05 ） , but no significant difference was found between experimental group and control group （ P 〉 0.05 ）. Pg was significantly associated with probing depth （PD） , sulcus bleeding index （SBI） and fasting blood-glucose level （P 〈 0.05 ）. Pi was closely related with PD, SBI, FBG and oral glucose tolerance test 2h blood-glucose（OGTY2hBG） （P 〈0.05）. Aa and Fn was corre- lated with SBI and PD （P 〈 0.05 ）. Tf was closely related with PD, SBI and FBG （P 〈 0.05 ）. CONCUSION ： The detection rates of putative periodontal pathogens in children with prediabetes and gingivitis, and in children with oeri-odontitis are significantly higher than in healthy children, indicating that blood glucose control and plaque control is im- portant in maintaining oral health.

关 键 词：Ⅱ型糖尿病 牙龈炎 PCR 

分 类 号：R780.2[医药卫生—口腔医学]