急性白血病患儿CD4^+CD25^+调节性T细胞和NK细胞免疫作用探讨  

Study of CD4^+CD25^+ regulatory T cells and NK cells in peripheral blood of children with acute leukemia

作　　者：史红鱼[1] 郝国平[1] 王晓欢[1] 常虹[1] 赵志华[1] 朱雷[1] 

机构地区：[1]山西省儿童医院血液科,太原030013

出　　处：《中国小儿血液与肿瘤杂志》2012年第4期177-179,185,共4页Journal of China Pediatric Blood and Cancer

摘　　要：目的观察外周血CD4+CD25+调节性T细胞(CD4+CD25+Treg)及自然杀伤细胞(na-ture killer cell,NK)在白血病患儿及非白血病患儿的不同,了解白血病患儿的免疫状态,探讨CD4+CD25+Treg细胞及NK细胞在小儿急性白血病肿瘤免疫中的意义。方法以流式细胞术检测急性白血病初诊患儿及非白血病患儿各30例的外周血CD4+CD25+Treg细胞、NK细胞的数量及比例。结果外周血CD4+CD25+CD127-细胞占CD4+T细胞的比例白血病组为(11.45±1.41)%,显著高于对照组为(6.98±1.09)%(P<0.05)。而NK细胞数量白血病组为(5.13±2.97)%,显著低于对照组为(15.06±3.91)%(P<0.05)。结论 (1)急性白血病患儿外周血CD4+CD25+Treg细胞数量升高,NK细胞数量降低,表明急性白血病患儿NK细胞免疫功能处于抑制状态。CD4+CD25+Treg细胞可能在白血病的发生、发展中起一定作用。(2)通过检测CD4+CD25+CD127-T细胞可较好的反映CD4+CD25+Treg细胞的比例,简便可行、重复性好、检测结果准确、可靠。Objective To investigate the expression features of CD4^ ＋ CD25 ^＋ regulatory T （Treg） cells and NK cells in peripheral blood （ PB ） of children with acute leukemia （ AL）, and observe the immune status of patients. The functions of CD4^ ＋ CD25 ^＋ Treg cells and NK cells in leukemia immunity were also explored. Methods The numbers and proportions of CD4^ ＋ CD25 ^＋ Treg cells and NK cells were detected by flow cytometry in PB samples of 30 newly diagnosed （ND） AL patients as well as 30 non-AL children. Results The average proportions of CD4^ ＋ CD25 ^＋ CD127 - cells to the CD4 ^＋ T cells in ND AL patients （11.45 ± 1.41 ）% were higher than those in non-AL children（6.98 ± 1.09）%. The NK cell counts in AL patients （5.13 ± 2.97） % were lower than those in non-AL children （15.06 ± 3.91% ） （P 〈 0.05）. Conclusion （1） The expresison features of Treg cells （increased） and NK cells （decreased） in AL patients indicated that the function of NK cells was suppressed. Treg cells may play a certain role in leukemogenesis, and are involved in the down-regulation of NK cells. （2） The application of CD4^ ＋ CD25 ^＋ CD127-on the detection of Treg cells is simple, reproductive and accurate, which could better reflect the proportions of CD4^ ＋ CD25 ^＋ Treg ceils.

关 键 词：CD4+CD25+CD127-调节性T细胞 NK细胞 儿童 急性白血病 

分 类 号：R733.71[医药卫生—肿瘤]