三种慢性胰腺炎大鼠模型的观察与比较  

Evaluation and Comparison on Three Chronic Pancreatitis Rat Models

作　　者：赵航[1] 黄丹丹[2] 董育玮[1] 张汝玲[1] 周慧[1] 

机构地区：[1]上海交通大学附属第一人民医院消化内科,上海200080 [2]第二军医大学附属长海医院急诊科,上海200433

出　　处：《实验动物与比较医学》2012年第4期270-273,共4页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金项目（81000165）

摘　　要：目的观察与比较雨蛙素注射、胰胆管结扎及精氨酸注射三种慢性胰腺炎造模方法。方法将大鼠随机分入雨蛙素注射（Cerulein）组，胰胆管结扎（Ligatjon）组，精氨酸注射组（Arginine）。采取相应方法制作慢性胰腺炎动物模型，在造模后3d、7d、14d三个时间点检测血淀粉酶、丙氨酸氨基转氨酶、血糖水平，ELISA法检测血清TNF—a，IL—1b水平，获取胰腺组织标本，对胰腺水肿、炎症、出血、腺泡坏死进行病理评分，应用VanGieson染色法对胰腺胶原纤维染色。RT—PCR法检测TGF_b表达。结果造模后各时间点，各组丙氨酸氨基转氨酶、血糖、TNF-a、IL-1b水平无明显差异，注射后3d，Cerulein组胰腺病理评分明显低于Ligation组和Arginine组。三组均可见胰腺组织胶原染色。Cerulein组3d时TGF-b表达低于Ligation组。结论三种造模方法各有优劣，但精氨酸注射法简便易行。Objective To observe and compare three chronic pancreatitis rat models including cerulean injection, pancreatic-bile duct ligation and L-arginine injection. Methods The rats were randomly divided into Cerulein group, Ligation group and Arginine group respectively. Chronic pancre-atitis were induced by intraperitoneal injection or surgery according to the grouping. Blood amylase, ALT, glucose, TNF-a and IL-lb were detected on 3 d, 7 d and 14 d after modeling. Histopathological scores of pancreas were evaluated and collagen in pancreas was stained with Van Gieson method. Expression of TGF-b in pancreas were detected by RT-PCR method. Results There was no significant difference in serum ALT, glucose, TNF-a and IL-lb levels of three groups. On 3d time point, histopathological scores of Cerulein group were significantly lower than that of Ligation group and Arginine group. Reticulate red stained collagen could be found in all three groups. Expression of TGF-b in pancreas of Cerulein group was significantly lower than that of Ligation group. Conclusions Three chronic pancratitis models all have their advantages and disadvantages, and method using arginine in chronic pancreatitis model induction is more convenient.

关 键 词：慢性胰腺炎 动物模型 大鼠 雨蛙素(Cerulein) 结扎(Ligation) 精氨酸(Arginine) 评价 

分 类 号：R576[医药卫生—消化系统] R-332[医药卫生—内科学]