大鼠胫骨移植癌细胞疼痛模型评价方法的探讨  

Evaluations on Rat Model of Tibia Cancer Pain with Bone Mineral Density

作　　者：缪建成 赵莹 陈国强 乔伟伟[3] 赵丽亚 金益 刘大海 赵先哲[3] 

机构地区：[1]上海西普尔-必凯实验动物有限公司,上海201203 [2]上海实验动物研究中心,上海201203 [3]复旦大学,上海200433

出　　处：《实验动物与比较医学》2012年第4期290-294,299,共6页Laboratory Animal and Comparative Medicine

基　　金：上海市科学技术委员会创新行动计划项目（N0.09140901200）

摘　　要：目的借助影像学方法，通过骨密度（BMD）指标对大鼠骨癌痛模型进行评价，探索该评估方法的可行性。方法选择雌性SD大鼠36只，随机分为假手术．生理盐水组（PN）、癌细胞（模型）-生理盐水组（wN）及癌细胞（模型）-伊班膦酸钠组（WP），各12只。大鼠经胫骨注射Walker256大鼠乳腺癌细胞，制备胫骨癌痛模型。对各组进行自发痛评分、机械刺激痛觉超敏检测、X射线成像、骨密度测量及病理组织学观察。结果骨密度检测可以区分各模型组的差异，且检测结果与行为学、组织学等检测方法相符。结论骨密度指标可以用于评价大鼠胫骨癌痛模型。Objective To evaluate the rat tibia cancer pain model with bone mineral density by iconography. Method Thirty six female Sprague-Dawley （SD） rats were randomly divided into a control group and two model groups with 12 rats in each. Rats from model groups were received intra- tibia injection of syngeneic Walker 256 rat mammary gland carcinoma cells. Series of tests were carried out including spontaneous pain score, mechanical stimuli pain hypersensitivity test, X-ray imaging, bone mineral density test and histopathology observation. Result Bone mineral density test could make the difference between each group, and the result of BMD test was the same as other tests. Conclusion Bone mineral density （BMD） can be used to evaluate rat tibia cancer pain model.

关 键 词：胫骨癌痛模型 大鼠 WALKER 256 骨密度(BMD) 

分 类 号：R738.1[医药卫生—肿瘤] R-332[医药卫生—临床医学]