山楂叶总黄酮的理化性质研究  

Study on Physicochemical Properties of Hawthorn Leaves Flavonids

作　　者：侯桂兰[1] 高敏[2] 童丽姣 王鑫波 石森林[2] 

机构地区：[1]浙江省肿瘤医院,浙江杭州310022 [2]浙江中医药大学药学院,浙江杭州310053 [3]临安市中医院,浙江临安311300

出　　处：《中华中医药学刊》2012年第8期1766-1768,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技资助项目(2008CA090)

摘　　要：目的:考察山楂叶总黄酮的平衡溶解度、油水分配系数等理化常数,研究温度、pH值等对其水溶液稳定性的影响。方法:测定山楂叶总黄酮在不同介质中的平衡溶解度与不同pH条件下的表观油/水分配系数(Papp),以10h后的药物含量变化率为指标考察介质、温度、pH对其稳定性的影响。结果:山楂叶总黄酮在蒸馏水、肠溶液、0.1%盐酸溶液、甲醇、乙醇、pH5.0 PBS、pH 6.5 PBS、pH 7.0 PBS、pH 7.4 PBS、pH 9.0 PBS中的平衡溶解度分别为2.81、5.07、1.62、60.76、31.57、3.78、4.54、8.74、9.24、9.15mg/mL;山楂叶总黄酮在pH 3.0、4.0、5.0、6.0、7.0、8.0条件下的油/水分配系数,分别为0.256、0.332、0.371、0.385、0.235、0.234。结论:山楂叶总黄酮的Papp值均随着pH增大而增大,在pH 6.0与pH 7.0间发生突变,pH 6.0时达最大值,之后随着pH增大而减小;较高pH条件对山楂叶总黄酮水溶液稳定性影响较大。Objective：To investigate the physicochemical constants just like equilibrium solubility and apparent parti- tion coefficients（ papp）, and study the effects of temperature and pH value on the stability of hawthorn leaves flavonids. Method：The equilibrimn solubility of hawthorn leaves flavonids in various medium and the papp of hawthorn leaves fla- vonids under different pH conditions can be determined by UV - Vis, the effects of temperature and pH value on the sta- bility of solution can be investigated by taking change ratios of drug content in lOh as detection index. Result：It can be known the equilibrium solubility of hawthorn leaves flavonids in distilled water, intestinal solution, O. 1% hydrochloric acid, methanol, ethanol, PBS with pH value 5.0, PBS with pH 6.5, PBS with pH 7.0, PBS with pH 7.4, PBS with pH 9.0 were 2.81,5.07,1.62,60.76,31.57,3.78,4.54,8.74,9.24,9.15 mg/mL, respectively; and its oil/water partition co- effieient in pH 3.0,4.0,5.0,6.0,7.0,8.0 were 0. 256,0. 332,0. 371,0. 385,0. 235,0. 234 ,respectively. Conclusion： The papp of hawthorn leaves flavonids was increased by the pH value, at pH 6.0,with the maximum, then decreased with the increasing pH value ; the stability of hawthorn leaves flavonids solution was apprantly inflused by high pH value （ pH 9.0）.

关 键 词：山楂叶总黄酮磷脂复合物 平衡溶解度 表观油/水分配系数 稳定性 

分 类 号：R222.19[医药卫生—中医基础理论]