6例姑息治疗期大肠癌血瘀证血清蛋白质组学探索性研究  

Exploratory Study of Serum Proteomics on Blood-Stasis Syndrome of Colorectal Carcinoma Palliative Therapy

作　　者：赵海燕 屠德敬 夏溪[3] 卢德赵[4] 郭勇[4] 

机构地区：[1]杭州市江干区中医院,浙江杭州310016 [2]苍南县中医院,浙江苍南325800 [3]慈溪市人民医院,浙江慈溪315300 [4]浙江中医药大学,浙江杭州310006

出　　处：《中华中医药学刊》2012年第8期1777-1779,I0008,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划重大项目(2007ZA007)

摘　　要：目的:从蛋白质组的差异表达角度揭示大肠癌姑息治疗期血瘀证的分子生物学基础。方法:6例患者血清以双向凝胶电泳分离后经不同波长光激发扫描得到不同样品的蛋白质组图谱,经DeCyder Image QuantTMV6.5软件分析,选择血瘀组与健康组和气血组相差达1.15倍以上的蛋白作进行质谱鉴定。结果:最终确定了10个差异蛋白质有,其中IL-8、载脂蛋白A-I前体、维生素D结合蛋白、DJ-1基因、β-GDP解离抑制因子均上调,纤维结合蛋白下调。结论:大肠癌血瘀证的产生可能与炎症相关蛋白和肿瘤相关蛋白的上调、与血管完整性相关蛋白的下调有关。Objective ：6 cases of blood -stasis syndrome were studied to explore the essence on molecular biology in palliative therapy by analyzing different expressions of serum proteomics. Methods ： Serum protein of 6 cases on blood - stasis syndrome of palliative therapy was separated by 2D gel electrophoresis （2 - DE）. The gels were respectively imaged and analyzed by DeCyder Image QuantTM V6.5 in blood - stasis syndrome, normal group, deficiency of Qi and blood com- parison group. Results ： 10 proteins were definited in blood - stasis syndrome of the cases as following ： the expressions of Interleukin - 8, Apolipoprotein A - I, Vitamin D - binding protein precursor, DJ - 1 and β - GDP increased, meanwhile, Fibronectin decreased. Conclusions ： The blood - stasis syndrome of palliative therapy in colorectal carcinoma could be at- tributed to proteins,which had a increasing correlation with tumor and inflammation and a decreasing correlation with the integrity of blood vessels.

关 键 词：大肠癌 蛋白质组学 血瘀证 

分 类 号：R735.34[医药卫生—肿瘤]