阿伐他汀诱导人胆管癌QBC939细胞凋亡的研究  

Effects of Atorvastatin on the proliferation and apoptosis in human cholangiocarcinoma QBC939 Cells

作　　者：郑龙志[1] 陈有挺[2] 石铮[2] 

机构地区：[1]福建省莆田学院附属医院普通外科,莆田351100 [2]福建医科大学附属第一医院普通外科,福州350005

出　　处：《江西医药》2012年第6期480-482,共3页Jiangxi Medical Journal

摘　　要：目的观察阿伐他汀(ATV)对人胆管癌QBC939细胞增殖及凋亡的影响。方法应用细胞培养技术培养QBC939细胞,经不同浓度的阿伐他汀处理后,以MTT法检测QBC939细胞的存活率,通过光镜、倒置显微镜及琼脂糖凝胶电泳检测胆管癌细胞凋亡的形态学特征、生化学特征。结果在阿伐他汀的作用下,QBC939细胞的生长、增殖变的相对缓慢,凋亡的细胞出现膜小泡、凋亡小体等特征性改变;DNA电泳呈现典型的梯状条带。结论阿伐他汀可抑制胆管癌细胞的生长、增殖,并可诱导细胞发生凋亡,且其作用呈剂量效应关系。Objective To investigate the effects of Atorvastatin on the proliferation and apoptosis in human cholangiocarci- noma QBC939 cells. Methods The human cholangiocarcinoma QBC939 cell line was incubated with different concentrations of a- torvastatin for 48h. MTT assay for cell growth and viability, Cell apoptosis was ascertained by cell morphology under light-,and inverted microscope,and DNA electrophoresis. Results Atorvastatin can inhibits growth and proliferation of QBC939 cells,and al- so induces cell apoptosis. Conclusion Atorvastatin can inhibits proliferation and induce apoptosis of human eholangioearcinoma QBC939 cells.

关 键 词：阿伐他汀 QBC939细胞 肿瘤细胞增殖 细胞凋亡 

分 类 号：R735.8[医药卫生—肿瘤]