杜仲叶提取物抗氧化作用与总黄酮含量的相关性研究  

Study on the Relativity of Antioxidant Activity and the Flavonoid Concentration of the Eucommia Leaf Extraction

作　　者：向灿辉[1] 邓镇涛[1] 王文君[1] 陈阳[1] 孙志勇[1] 陈开龙[1] 

机构地区：[1]遵义医学院珠海校区生物工程,珠海519041

出　　处：《食品工业》2012年第8期1-5,共5页The Food Industry

基　　金：贵州省科技厅项目(黔科合J字[2009]2291);贵州省教育厅基金项目(黔教科2010042)

摘　　要：采用二苯代苦味肼基自由基(DPPH.)和铁离子还原/抗氧化能力(FRAP)测定法,研究了杜仲叶提取物,芦丁的抗氧化活性,并同VC进行了比较。结果表明杜仲叶提取物具较强的抗氧化能力,抗氧化能力与总黄酮含量密切相关,在一定浓度范围内成线性关系,对DPPH.有较强的清除作用,半抑制量EC50为0.022mg/mL,各样品清除DPPH.能力为:VC>芦丁>杜仲叶提取液;在FRAP模型中,对铁离子的还原能力强度顺序为VC>杜仲叶提取物>芦丁;杜仲叶提取物抗氧化速度小于VC大于芦丁。Comparing with VC,the antioxidant capacities of Eucommia leaf extraction and Rutin was analyzed by two methods,namely,1,1-diphenyl-2-picrylhydrazyl（DPPH·） radical scavenging and ferric reducing antioxidant power（FRAP） assay.The results showed that Eucommia leaf extraction had high antioxidant activity,and the antioxidant capacity of Eucommia leaf extraction was closely correlated with the concentration of flavonoids.The DPPH· free radical scavenging activity of Eucommia leaf extraction was lower than that of VC and Rutin,the EC50（50% the initial DPPH· concentration） of Eucommia leaf extraction was 0.022 mg/mL.But the ability to reduce ferric ions of Eucommia leaf extraction was lower than that of VC but higher than that of Rutin.The speed of antioxidant of Eucommia leaf extraction was higher than that of Rutin,and lower than that of VC.

关 键 词：杜仲叶提取物 总黄酮 芦丁 抗氧化 相关性 

分 类 号：TQ461[化学工程—制药化工]