白术茯苓汤及水煎液提取分离组分对脾气虚大鼠血管活性肠肽受体亲和力的影响  

Effect of Baizhu Fuling Decoction and its separative components extracted from water solution on VIP-R appetency of rats with spleen Qi deficiency

作　　者：谢磊[1] 贾波[1] 罗云波[1] 祝捷[1] 彭成[1] 彭腾[1] 张丰华[1] 欧晓红[2] 

机构地区：[1]成都中医药大学,成都610075 [2]四川大学华西医院,成都610036

出　　处：《中华中医药杂志》2012年第8期2186-2188,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.30572296)~~

摘　　要：目的:观察白术茯苓汤及其水煎液提取分离组分对脾气虚大鼠血管活性肠肽(VIP)受体亲和力的影响。方法:以耗气破气加饥饱失常法复制脾气虚大鼠模型,采用放射性配基结合分析法检测各组VIP受体最大结合容量(RT)和受体亲和常数(Kd)。结果:各组RT无统计学差异;与空白组比较,模型组Kd值升高(P<0.01);与模型组比较,总多糖、络合物组Kd值呈下降趋势,白术内酯Ⅲ组Kd值呈升高趋势,但皆无统计学意义;白术茯苓汤组、组分配伍组Kd值显著降低(P<0.05)。结论:白术茯苓汤及组分配伍组能提高VIP受体亲和力;不同组分对Kd的效应具有一定差异性,配伍则显示可能具有协同增效作用。Objective：To observe the effects of Baizhu Fuling decoction and its separative components extracted from water solution on VIP-R appetency of rats with Spleen Qi deficiency.Methods：To copy the models of rats with spleen Qi deficiency with the method of exhaustion,dissipating stagnant Qi and irregular diet,radioactive ligands combination analysis was used to test each groups RT and Kd of receptor affinity of VIP.Results：RT value in all groups had no significant difference.Compared with blank group,Kd value in model group was higher（P〈0.01）.Compared with model group,Kd value of high dosage total polysaccharide,complex compound group was on a declining curve,while its value of Rhizoma Atractylodis Macrocephalae lactone Ⅲ high dosage group was of rising tendency,which were no significant difference.Baizhu fuling decoction and high dosage group of component compatibility could lower Kd Value（P0.05）.Conclusion：Baizhu fuling decoction and component compatibility group could raise receptor affinity of VIP;Kd effects of different groups possessed differentiation,and compatibility probably had synergistic effect.

关 键 词：白术茯苓汤 组分配伍 放射性配基结合分析法 脾气虚证 血管活性肠肽受体亲和力 

分 类 号：R285.5[医药卫生—中药学]