Bcl-2／Bax在百草枯中毒鼠肺组织中的表达及三七总皂苷的保护作用  

Expressions of apoptosis gene Bcl - 2 and Bax in the lung of paraquat poisoning rats and protection of PNS

作　　者：王晔 曲艳 王南 郝丽 王旭 

机构地区：[1]昆明市延安医院急诊科,云南昆明650051

出　　处：《中国急救医学》2012年第8期714-717,共4页Chinese Journal of Critical Care Medicine

基　　金：云南省教育厅科学研究基金资助项目（062168C）

摘　　要：目的探讨急性百草枯（PQ）中毒鼠肺组织病理损伤变化和肺组织中抗凋亡基因（Bcl-2）、促凋亡基因（Bax）的表达及三七总皂苷（PNS）的保护作用。方法150只SD雄性鼠分为正常对照组（C组，30只）、百草枯中毒组（PQ组，60只）及三七总皂苷保护组（PNS组，60只）。PQ组和PNS组一次性灌胃PQ25mg／kg染毒，C组予等体积生理盐水灌胃。其中PNS组于染毒前15min以PNS50mg／kg阴茎静脉注射保护，以后每日1次给药直至处死前；PQ组、c组分别在同时间点予与等体积生理盐水。观察各组大鼠在中毒后6h、12h、1d、3d、5d、7d肺组织病理改变，采用反转录-聚合酶链反应（RT-PCR）方法测定鼠肺组织Bcl-2和BaxmRNA的表达。结果PNS组Bcl-2mRNA的表达在6h、12h及1d各亚组高于相应点PQ组，差异有统计学意义（P〈0．05）；PQ组BaxmRNA的表达在6h、12h、1d及3d各亚组高于相应点PNS组，差异有统计学意义（P〈0．05）；PQ组Bcl-2／Bax比例为促凋亡的Bax基因占优势；PNS组Bcl-2／Bax比例亦为促凋亡的Bax基因占优势，但这种优势不如PQ组明显。PQ组肺病理损伤评分在6h、12h、1d及3d各亚组均高于PNS相应组，差异有统计学意义（P〈0．05），C组Bcl-2、BaxmRNA几乎不表达，肺组织病理大致正常，与PQ组及PNS组相比差异有统计学意义（P〈0．00）。结论Bcl-2／Bax基因不均衡表达参与PQ中毒所致肺损伤，PNS对百草枯中毒所致肺损伤有保护作用。Objective To explore the expressions of apoptosis gene Bel - 2 （B - cell leukemia - 2） and Bax （ bcl - xl/bcl - 2 - associated death promoter） in lung of paraquat poisoning rats and the protection of Panax Notoginseng Saponins （PNS）. Methods One hundred and fifty SD male rats were randomly divided into 3 groups ： normal control group （ C 30 rats ） , paraquat poisoning group （PQ 70 rats ） and drug intervention group （PNS 70 rats ）. The PQ and PNS group rats were given paraquat by gastric perfusion （25 mg/kg） ; The C group rats were given saline by gastric perfusion. The rats in PNS group were protected by intravenous injections of penis with PNS （50 mg/kg） 15 rain before exposure of paraquat, once a day until killed. The PQ and C group rats were given saline. Histological changes of lung tissues under light microscope were observed. And the expression of Bcl - 2 and Bax mRNA in lung tissues of rats were measured by RT - PCR at different time of 6 h,12 h,1 d,3 d and 7 d after poisoning. Results The relative magnitude expression of Bcl -2 in PNS （6 h）, PNS （12 h） and PNS （ 1 d） subgroups were significantly higher as compared with the PQ group （ P 〈 0.05 ）. The relative magnitude expression of Bax in PQ （6 h, 12 h, 1 d,3 d） subgroups were significantly higher as comparedwith PNS group. The pathological scores of lung injury in PQ （6 h, 12 h, 1 d, 3 d） sub groups were significantly higher compared with PNS group. Conclusion The aggravation of lung injury induced by paraquat in mice was related to the down - regulation of apoptosis gene Bcl - 2 expression and up - regulating apoptosis gene Bax expression in lung tissue. PNS can effectively improve the balance of Bcl - 2/Bax in lung tissue and reduce the pathological changes of lung tissue.

关 键 词：百草枯(PQ) 急性肺损伤(ALI) 三七总皂苷(PNS) B细胞淋巴瘤/白血病-2 促凋亡基因BAX 

分 类 号：R361.3[医药卫生—病理学]