双氢青蒿素对骨肉瘤细胞143B影响的研究  

Dihydroartemisinin induces apoptosis in osteosarcoma cells 143B

作　　者：刘跃亮[1] 罗进勇[1] 曾照芳[1] 宋涛[1] 

机构地区：[1]重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆400016

出　　处：《激光杂志》2012年第4期68-69,共2页Laser Journal

摘　　要：目的:研究双氢青蒿素(Dihydroatemisinine,DHA)对入骨肉瘤细胞143B的影响及所导致的形态学变化。方法:采用人骨肉瘤143B细胞株,通过设置对照组和低浓度、中浓度、高浓度的DHA组,H.E染色观察Trandwell小室穿梭能力;划痕愈合实验观察迁移能力;Hoechst33258荧光染色检测细胞凋亡;MTT法检测细胞增殖抑制能力。结果:DHA表现出明显地抑制人骨肉瘤143B细胞的迁移、增殖以及促进凋亡(P<0.05),且伴随浓度的递增和时间的延长,抑制作用更明显。结论:DHA具有较强的抗入骨肉瘤作用,其机制可能与DHA诱导骨肉瘤细胞凋亡,并抑制其增殖有关。Objective： To study dihydroartemisinin in human esteosarcoma cells 143B and the resulting impact of morphological changes. Method：using human osteosarcoma 143B cell line, by setting the control group, low cencentrations, concentration, high concentrations DHA group, H. E staining Trandwell chamber shuttle capacity; experimental observation of scratch healing migration; Hcechst 33258 staining to detect apoptesis; MTT cell proliferation assay suppression. The results showed significantly inhibit DHA in human osteosarcema 143B cell migration, proliferation and promote apoptesis （ P 〈 0.05 ）, and with increasing concentration and time extension, the trend is more obvious. Conclusion： DHA has strong anti - osteosarcoma effects, its mechanism may be related to induction of apoptosis in osteosarcoma cells, and inhibition of proliferation.

关 键 词：双氢青蒿素 人骨肉瘤细胞 细胞凋亡 细胞增殖 

分 类 号：R738.1[医药卫生—肿瘤]