β-catenin和表达cyclin—D1在散发性甲状旁腺瘤的  

Expression of β-catenin and cyclin-D1 in sporadic parathyroid adenomas

作　　者：钟春林[1] 杨治力[2] 伍波[2] 邓先兆[2] 康杰[2] 樊友本[2] 

机构地区：[1]苏州大学医学部,215006 [2]上海交通大学附属第六人民医院普外科 上海交通大学甲状腺疾病诊治中心,200233

出　　处：《中华内分泌外科杂志》2012年第4期221-224,共4页Chinese Journal of Endocrine Surgery

摘　　要：目的研究β-一连环蛋白（β-catenin）及细胞周期蛋白D1（cyclin-D1）在散发性甲状旁腺瘤中的表达及其临床意义。方法应用免疫组化、荧光定量PCR（RT—PCR）检测20例散发性甲状旁腺瘤、10例甲状旁腺增生、8例正常甲状旁腺组织中β-catenin、cyclin—D1的表达。结果免疫组化显示：β-catenin在正常甲状旁腺组织、甲状旁腺增生及散发性甲状旁腺瘤胞膜中的表达强度呈下降趋势，而在胞质和细胞核内逐渐增强；cyclin-D1在腺瘤组和增生组的表达明显高于正常组（P〈0．05），而腺瘤组与增生组比较差异无统计学意义（P〉0．05）；β-catenin异常表达与cyclin-D1的高表达在散发性甲状旁腺瘤中存在显著相关性（P〈0．05）。荧光定量PCR分析显示：cyclin—D1mRNA在腺瘤组织和正常甲状旁腺组织中的表达分别为2．36±1．12和1．50±1．03（P〈0．05），β-cateninmRNA在腺瘤组织和正常甲状旁腺组织中的表达分别为1．02±0．45和0．88±0．56（P〉0．05）。结论β—catenin的异常表达激活cyclin—D1引起细胞增殖和分化失控，可能是散发性甲状旁腺瘤发生机制之一。Objective To study the expression of β-catenin and cyclin-D1 in sporadic parathyroid adenomas and the clinical significance. Methods Immunohistochemistry and RT-PCR were used to examine the expression of β-catenin and cyclin-D1 in 20 cases of sporadic parathyroid adenomas, 10 cases of parathyroid hyperplasia tissues and 8 cases of normal parathyroid tissues respectively. Results The results of immunohistochemistry showed that the expression of β-catenin was decreased on cell membrane of normal parathyroid tissues, parathyroid hyperplasia tissues and sporadic parathyroid adenomas, and was increased in the cytoplasm and the nucleus. The expression of cyclin-D1 was significantly higher in the adenoma group and hyperplasia group than in the normal group（ P 〈 0. 05 ）. The difference had no statistical significance in terms of cyclin-D1 expression between the adenoma group and the hyperplasia group（P 〉 0. 05 ）. The abnormal expression of β-catenin was significantly correlated with the overexpression of cyclin-D1 in sporadic parathyroid adenomas （ P 〈 0. 05 ）. RT-PCR analysis showed that the expression of cyclin-D1 mRNA was 2. 36 ± 1.12 vs 1.50 ± 1.03（P 〈0. 05）, and the expression of β-catenin mRNA was 1.02 ± 0.45 vs 0. 88 ± 0. 56 （ P 〉 0. 05 ） in adenomas and normal parathyroid tissues respectively. Conclusion The abnormal expression of β-catenin activates cyclin-D1 and thus leads to the uncontrolled cell proliferation and differentiation, which may be one of the mechanisms of the occurrence of sporadic parathyroid tumors.

关 键 词：散发性甲状旁腺瘤 细胞周期蛋白D1 Β-连环蛋白 

分 类 号：R736.2[医药卫生—肿瘤]