HPLC法测定不同储藏条件及时期雪莲果块根中绿原酸含量  

HPLC Analysis of Chlorogenic Acid Content in Yacon Tubers under Different Storage Conditions and Storage Time

作　　者：魏军香[1] 张跃进[1] 梁宗锁[1] 任怀远[2] 高锡忠[2] 

机构地区：[1]西北农林科技大学生命科学学院,中药指纹图谱与天然产物国家地方联合中心,陕西杨凌712100 [2]陕西略阳县农业技术推广中心,陕西略阳724300

出　　处：《西北农业学报》2012年第7期118-122,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家"十一五"科技支撑项目(2008BAD98B08)

摘　　要：采用超声波提取、高效液相色谱法测定两种储藏条件下随储藏时间延长雪莲果块根中绿原酸含量的动态变化,并对块根中绿原酸提取方法进行优化,同时考察HPLC方法的精密度和准确度。结果表明,提取溶剂采用φ=50%甲醇、溶剂量50倍、提取时间40min为最佳条件。方法精密度和准确度表明,用乙腈(φ=0.1%)磷酸水溶液V(乙腈)∶V(磷酸)=15∶85洗脱、流速1.0mL·min-1、柱温30℃、检测波长327nm的色谱条件可靠。用此方法测定绿原酸含量结果表明,两种储藏条件下随储藏时间延长,绿原酸含量均呈先缓慢下降后加剧减少的变化趋势。建议以绿原酸为利用目标的应选用短期储存,即以低温储藏85d以内为佳。The dynamic change of chlorogenic acid content in yacon tubers under two different storage conditions was analyzed by HPLC and ultrasonic extraction.The extraction technology was optimized and tests were conducted to study the precision and accuracy of the method.Results indicated that the optimum condition for ultrasonic extraction of chlorogenic acid in yacon tubers was as follow： φ=50% methanol as the dissolving solution,50 fold solvent dosage,extracted for 40 min.The precision and accuracy test result of HPLC method showed that the chromatographic conditions were reliable.The contents of chlorogenic acid under two storage conditions were determined by this method and the result showed that content of chlorogenic acid declined gradually at beginning but rapidly afterwards as the extension of the storage time,so the best storage condition and time is within 85 days under 4 ℃ when chlorogenic acid in tubers is the main pursuit.

关 键 词：雪莲果 绿原酸 储藏条件 超声提取 高效液相色谱(HPLC) 

分 类 号：R284[医药卫生—中药学]