尿脱落细胞端粒酶活性检测在膀胱癌早期诊断中的价值  

Telomerase Activity Detection in Urine Exfoliated Cells for Early Diagnosis of Bladder Carcinoma

作　　者：高兴成[1] 章咏裳[1] 周四维[1] 叶章群[1] 

机构地区：[1]同济医科大学附属同济医院泌尿外科,430030

出　　处：《实用癌症杂志》2000年第4期405-407,共3页The Practical Journal of Cancer

基　　金：国家自然科学基金! (3970 0 1 69);上海市教委重点学科项目

摘　　要：目的　探讨膀胱癌早期诊断的新的无创性检查方法。方法　应用端粒酶活性PCR ELISA法和细胞学方法对 5 3例膀胱癌、2 6例良性膀胱疾病和 17例正常人尿脱落细胞进行检测 ,并对 2种方法检测的阳性率以及病理检查结果进行比较。结果　PCR ELISA法检测膀胱癌患者尿脱落细胞端粒酶活性的总阳性率 ( 64 .2 % )明显高于尿细胞学检查的总阳性率 ( 3 9.6% ) ;在早期、低级别膀胱癌 (T1、G1)中 ,端粒酶活性阳性率分别为 61.1%、5 5 .6% ,尿细胞学阳性率分别为 2 4.2 %、15 .4% ,均具有非常显著性差异 (P <0 .0 1)。结论　PCR ELISA法检测端粒酶活性可以作为膀胱癌早期诊断的无创性检查方法。Objective In order to search a new early diagnostic method for bladder carcinoma.Methods The samples of exfoliated cells in urine from 53 bladder carcinoma,26 non neoplastic bladder lesions and 17 normal volunteers were detected by Polymerase chain reaction Enzyme linked immunosorbent assay(PCR ELISA) for telomerase activity.Results Of the 53 bladder carcinoma specimens,64.2% was tested positive telomerase and 55.6% of the Grade 1 tumors was positive.By comparison,only 39.6% of the samples from these patients yielded cytological positive findings and only 15.4% of the Grade 1 tumors were diagnosed by cytology.The difference in overall detection rates(64.2% for telomerase vs 39.6% for cytology) was significant( P <0.01).Furthermore,in comparison with cytology for diagnosis of low grade bladder carcinoma(Grade 1),the difference was also significant( P < 0.01 ).Conclusion Telomerase activity detection would be a useful diagnostic marker for the early noninvasive identification of patients with bladder carcinoma.

关 键 词：膀胱癌 端粒酶 脱落细胞 早期诊断 

分 类 号：R737.140.4[医药卫生—肿瘤]