肺癌组织与细胞凋亡和增殖的关系  

Relationship between lung cancer and apoptosis and proliferation

作　　者：丁续红[1] 杨炯[1] 胡克[1] 吴小军[1] 李清泉[1] 

机构地区：[1]湖北医科大学附属第一医院呼吸内科,武汉430060

出　　处：《临床内科杂志》2000年第4期229-230,共2页Journal of Clinical Internal Medicine

摘　　要：目的　探讨肺癌与细胞凋亡和增殖的关系。方法　对 38例肺癌组织及 2 1例肺部良性组织 ,应用 TUNEL 技术对细胞凋亡情况进行检测 ,应用免疫组织化学法对增殖细胞核抗原(PCNA)等进行检测 ,并对细胞凋亡和 PCNA阳性细胞进行计数 ,分别定义凋亡指数和增殖指数。结果　肺癌组织中凋亡指数 [(3.73± 2 .42 ) % ]和增殖指数 [(13.86± 15 .0 5 ) ]%均高于肺部良性组织 [(0 .77± 0 .5 0 ) %、(0 .91± 3.19) % ],P <0 .0 1。结论　细胞凋亡和Objective To study the relationship apoptosis and proliferation lung cancer.Methods In 38 cancer cases and 21 benign respiratory cases, TUNEL (TdT dUTP nick and labelling) technique was applied to detect the apoptosis and the immunohistochemistry was applied to detect PCNA . The apoptotic index (AI) and mitotic index (MI)was defined.Results The AI and MI of lung cancer [(3.73±2.42)%,(13.86±15.03)%, respectively] were higher than those of benign respiratory cases[(0.77±0.50)%、(0.91±3.19)%, respectively, P<0.01].Conclusion Apoptosis and PCNA can be used as markers of lung cancer. [

关 键 词：肺肿瘤 增殖细胞核抗原 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]