微透析探针对杨树嫩茎细胞损伤的显微观察  

Microscopic Observation of Cell Damage on Poplars Spears by Microdialysis Probe

作　　者：孙若峥[1] 吴祥云[1] 姜国斌[2] 马金龙[2] 邓绍立[3] 刘思莹[1] 

机构地区：[1]辽宁工程技术大学环境科学与工程学院,辽宁阜新123000 [2]大连民族学院环境与资源学院,辽宁大连116600 [3]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《山西农业科学》2012年第8期811-814,818,共5页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(30872004);中央高校基本科研业务费资助项目(DC12010204;DC12010305);辽宁省重点农业攻关计划项目(2008207001)

摘　　要：利用微透析技术研究植物汁液成分时,所遇到的首要问题是其透析液来自质外体还是共质体。以冬季水培的吴屯杨和荷兰杨幼苗为材料,采用石蜡切片法,制作微透析探针插入杨树嫩茎后,对嫩茎受损部分横切面组织切片进行显微观察并加以探讨。结果表明,微透析探针的粗度约为杨树细胞直径20倍,探针对周围细胞造成一定程度的破坏,因此,探针插入杨树嫩茎后开始一段时间内收集的透析液将受到破损细胞液的污染,随着微透析时间的推移,逐渐地趋近于质外体汁液。对微透析技术可以获得植物质外体汁液从细胞显微结构变化方面予以解释,为微透析技术在植物质外体研究中的应用奠定细胞学基础。When we use microdialysis technique to study the composition of plant sap,the primary problem we encountered is whether the dialysis fluid came from the apoplast or symplast？ In this study,we used seedlings of winter hydroponic Populus.wutunenses and holland young as the material,and made the slice of cross-section tissue of spears damaged part after micro-dialysis probe inserted into the poplar spears with paraffin section method,to do microscopic observations and further study.The results showed that： the roughness of microdialysis probe was about 20 times greater than the diameter of poplar cells;probe caused a certain degree of damage to the surrounding cells;so the collecting dialysate would be contaminated by damaged cell fluid,after probe inserting poplar spears in a period of time,with the time passing ofmicrodialysis,dialysate was close to apoplastic sap.This study explained that the microdialysis technique could get the plant apoplast sap from the micro-structural changes of cells,providing a cytological basis for the application of the microdialysis technique in the plant apoplast.

关 键 词：微透析 杨树 组织切片 细胞破损 显微观察 

分 类 号：S718.4[农业科学—林学] S792.11