西宁地区耳鸣动物模型5-羟色胺水平变化  

The Changes of Blood 5-Hydroxytryptamine in Plateau Tinnitus Animal Model

作　　者：张英[1] 刘俊秀[2] 

机构地区：[1]青海大学附属医院耳鼻咽喉科,810001 [2]北京大学第三医院耳鼻咽喉科,100191

出　　处：《高原医学杂志》2012年第1期15-18,共4页Journal of High Altitude Medicine

基　　金：国家自然科学基金资助项目(项目编号:20035010)

摘　　要：目的:探讨水杨酸钠所导致高原地区耳鸣动物模型体内5-羟色胺(5-HT)浓度的变化。方法:将Wister大鼠随机分成腹腔内注射水杨酸钠诱导建立耳鸣组(n=10)和腹腔内注射生理盐水的空白对照组(n=10),利用高效液相色谱法测定大鼠血液中5-HT浓度的变化。结果:注射水杨酸钠组(350 mg/kg)血液中5-HT水平的显著升高,在给药后的2h升到最高,达到基础值得(168±14)%,给药后4小时恢复到基线水平,而生理盐水组未引起明显变化。结论:本研究表明耳鸣动物模型血液中5-HT水平的升高可能和耳鸣的产生有关。Objectives：To stud）, the changes of 5 -hydroxytryptamine （5 -HT） in tinnitus animal model and, whereby, maybe provide a better underslanding of neurological mechanism of salicylate - induced tinnitus. Methods：In our study, the Wister rats were randomLy divided into tinnitus group （ n = 10） with administration of salicylate （350mg/kg, i. p. ） and control group （n = 10） with administration of saline. The changes blood have been monitored by high performnance liquid chromatography. Results：The 5 - HT levels significantly increased after application of salicylate. The 5 - HT level increased to a maximum of 168% ±14%basal level in blood 2h level did not appear an of 5 -HT levels in rat after a y clear blood pplicatio changes n of salicylate and returned to the basal level after ap cation of saline. Conclusions ： These may be involved in the tinnitus generation around 4h, while the 5 - HT data suggest that the increases

关 键 词：水杨酸钠 耳鸣 血液 5-HT 高效液相色谱法 

分 类 号：R-332[医药卫生] R764.45