神经母细胞瘤患者尿液儿茶酚胺代谢物及血清癌胚抗原、神经烯醇化酶水平变化分析  

Analysis of Urinary Catecholamine Metabolites,Serum Carcinoembryonic Antigen and Neural Enolase in Neuroblastoma Patients

作　　者：杜同信[1] 方群[1] 王自正[1] 俞杨[1] 傅雷[1] 赵飞浪[1] 周俊山[2] 周峰[2] 

机构地区：[1]南京医科大学附属南京第一医院南京临床核医学中心,江苏南京210006 [2]南京医科大学附属南京第一医院神经内科,江苏南京210006

出　　处：《标记免疫分析与临床》2012年第4期226-228,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨尿中儿茶酚胺代谢物及血清癌胚抗原(CEA)、神经烯醇化酶(NSE)在神经母细胞瘤(NB)诊断和鉴别诊断中的应用价值。方法用高效液相色谱和化学发光免疫分析技术,分别对36例NB患者和30名正常对照者尿液儿茶酚胺代谢产物香草扁桃酸(VMA)、高香草酸(HVA)和血清CEA、NSE含量进行检测分析。结果 NB患者尿液VMA、HVA和血清CEA、NSE均显著高于正常对照组(P<0.01),VMA、HVA单项检测的阳性率分别为86.1%、75.0%,两项同时检测可提高到91.7%,结合NSE、CEA进行三项或四项联合检测,其阳性率可分别提高至94.4%和97.2%。结论尿液儿茶酚胺代谢物VMA、HVA和血清肿瘤标志物CEA、NSE水平变化对NB的诊断和鉴别诊断具有重要意义,这些指标的联合检测可大大提高检测的阳性率。Objective To investigate the value of detection of urinary catecholamine metabolites,serum carcinoembryonic antigen（CEA） and neural enolase（NSE） in the diagnosis and differential diagnosis of neuroblastoma（NB）.Method The urinary catecholamine metabolites including vanillylmandelic acid（VMA） and homovanillic acid（HVA）,and serum CEA and NSE levels in 36 neuroblastoma patients and 30 controls were detected by high-performance liquid chromatography and chemiluminescence assay respectively.Results The urinary catecholamine metabolites VMA,HVA,serum CEA and NSE in NB patients were significant higher than those in the control group（P0.01）.The positive rate of VMA and HVA in NB patients was 86.1% and 75.0 % respectively.The positive rate of combination detection of VMA and HVA was increased to 91.7%.The combined detection with serum NSE or CEA,the positive rate increased to 94.4%,while combined detection with both serum NSE and CEA,the positive was increased to 97.2%.Conclusion The detection of urinary catecholamine metabolites VMA,HVA,serum CEA and NSE level play an important role in the diagnosis and differential diagnosis of neuroblastoma.The combination detection could improve the positive rate.

关 键 词：神经母细胞瘤 高效液相 化学发光分析 肿瘤标志物 

分 类 号：R730.4[医药卫生—肿瘤]