致病性结核杆菌特异性IgG抗体ELISA检测方法的初步建立  

Establishment of ELISA for Detection of Specific IgG Antibodies of Pathogenic Mycobacterium tuberculosis

作　　者：王云龙[1,2] 周贺娟[1,3] 李玉林[2] 孙新城[3,4] 董彩文[3,4] 王国强 刘旺根 王继创 李恒思 程蕾 白晓静[2] 

机构地区：[1]郑州大学生物工程系,河南郑州450001 [2]郑州职业技术学院,河南郑州450001 [3]河南省生物工程技术研究中心,河南郑州450001 [4]郑州轻工业学院食品与生物工程学院,河南郑州450002

出　　处：《动物医学进展》2012年第8期27-30,共4页Progress In Veterinary Medicine

基　　金：河南省科技创新团队和郑州市科技创新团队项目

摘　　要：为建立鉴别致病性结核杆菌感染和卡介苗接种产生的IgG抗体,分别用结核杆菌的混合抗原TB1、TB2和PPD包被,以结核病患者血清为阳性对照,以PPD试验阴性健康人血清作为阴性对照,建立了TB1-ELISA、TB2-ELISA、PPD-ELISA三种检测结核杆菌抗体的方法。20份阳性标本检测结果显示,TB1-ELISA、TB2-ELISA、PPD-ELISA的阳性检出率分别为45%、50%、95%,100份健康人血清标本检测结果显示,TB1-ELISA、TB2-ELISA、PPD-ELISA的符合率为93%、90%、68%。研究工作为研发致病性结核杆菌感染IgG抗体检测试剂奠定了一定基础。We managed to establish an ELISA method to distinquish the IgG level of Mycobacterium tuber- culosis infected people from those vaccinated with BCG vaccine. Antigens of TB1, TB2 and PPD were coa- ted respectively. Positive controls are serum samples from Mycobacteriurn tuberculosis infected peoples and negative controls are serum samples from healthy peoples who are not infected and PPD test are negative. Three different ELISA methods used for detecting Mycobacteriurn tuberculosis IgG antibodies were crea- ted; they are TB1-ELISA, TB2-ELISA and PPD-ELISA. The coating and blocking buffer solutions and enzyme diluents were optimized. The result for twenty positive samples showed that the positive rates of TB1-ELISA, TB2-ELISA and TB3-ELISA is 45%, 50%and 95% ,respectively. The result for one hundred serum samples from healthy people showed that the coincidence rate of TB1-ELISA, TB2-ELISA and PPD- ELISA is 93%, 90%and 68% ,respectively. It laid foundation for the establishment of ELISA detection re- agents of IgG antibody of pathogenic Mycobacterium tuberculosis.

关 键 词：结核杆菌 IGG抗体 酶联免疫吸附试验 

分 类 号：S852.618[农业科学—基础兽医学]