正常和缺氧条件下永磁磁场对人脑微血管内皮细胞超氧化物歧化酶和丙二醛的影响  

Effects of permanent magnetic fields on superoxide dismutase and malondialdehyde in human microvascu- lar endothelial brain cells under normal and hypoxic conditions

作　　者：孟庆楠[1] 王益民[1] 王蕴华[2] 刘彦强[1] 孙秀岩[2] 

机构地区：[1]天津中医药大学中医工程研究所,天津300193 [2]天津中医药大学实验教学部

出　　处：《中华物理医学与康复杂志》2012年第8期570-572,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金资助（30873467）

摘　　要：目的观察不同强度永磁磁场对正常和缺氧条件下人脑微血管内皮细胞（HBMEC）氧化损伤的影响。方法实验分为对照组和8个不同强度（8．1mT、16．5mT、20．3mT、26．0mT、27．3mT、62．5mT、110．7mT、215．6mT）的永磁磁场组（加磁1组～加磁8组），在正常和缺氧两种条件体外培养人脑微血管内皮细胞，于72h后采用超氧化物歧化酶（SOD）法和丙二醛（MDA）法检测各组人脑微血管内皮细胞氧化损伤的变化情况。结果正常环境下，加磁1组到加磁6组的SOD值均略低于对照组，且加磁7组和加磁8组的SOD值又略高于对照组，但差异均无统计学意义（P〉0．05）；加磁各组的MDA值均高于对照组，其中仅加磁8组的MDA值与对照组比较，差异有统计学意义（P〈0．05）。在缺氧环境下，加磁1组、加磁2组、加磁4组、加磁7组、加磁8组的SOD值均低于对照组，而加磁3组、加磁5组、加磁6组的SOD值则高于对照组，但差异均无统计学意义（P〉0．05）；加磁1组、加磁2组、加磁4组的MDA值均高于对照组，加磁3组、加磁5组、加磁6组、加磁7组、加磁8组的MDA值则低于对照组，其中仅加磁7组的MDA值与对照组比较，差异有统计学意义（P〈0．05）。结论正常条件下，磁感强度215．6mT的永磁磁场对人脑微血管内皮细胞MDA有影响；缺氧条件下，磁感强度110．7mT的永磁磁场对人脑微血管内皮细胞MDA有影响。Objective To observe the effects of permanent magnetic fields with different magnetic inductions on oxidative damage to human brain microvascular endothelial cells （HBMECs） under normal and hypoxie conditions. Methods HBMECs were cultured in vitro under normal and hypoxie conditions, then divided into a control group and groups receiving magnetic induction at 8. 1, 16. 5, 20. 3, 26.0, 27. 3, 62. 5, 110. 7 and 215.6 inT. Changes in superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in each group were measured 72 b after exposure to the magnetic fields. Results Under normal conditions the SOD activities of the magnetic groups were not significantly different from that of the control group. Only the MDA content of the 215.6 mT group was statistically different （slightly higher） than that of the control group （P 〈 0.05 ）. Under hy- poxic conditions, there was again no statistically significant difference in SOD activity, but the MDA content of the 110.7 mT group was significantly lower. Conclusions Under normal conditions, a 215.6 mT permanent magnetic field has an effect on MDA in HBMECs; under hypoxie conditions a 110.7 mT permanent magnetic field has such an effect.

关 键 词：永磁磁场 人脑微血管内皮细胞 超氧化物歧化酶 丙二醛 

分 类 号：R74[医药卫生—神经病学与精神病学]