血管内皮生长因子C与细胞角蛋白19检测在Ⅰ期非小细胞肺癌中的临床研究  

Clinical Study of Vascular Endothelial Growth Factor-C and Cytokeratin 19 in StageⅠNon-small Cell Lung Cancer Patients

作　　者：王耀鹏[1] 王明钊[1] 罗宜人[1] 魏朝霞[1] 玄云鹏[1] 

机构地区：[1]青岛大学医学院附属医院胸外科,山东青岛266003

出　　处：《中国胸心血管外科临床杂志》2012年第4期385-389,共5页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目的探讨血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C)表达、细胞角蛋白19(cytokeratin 19,CK19)检测在Ⅰ期非小细胞肺癌(non-small cell lung cancer,NSCLC)患者生存分析中的意义。方法纳入青岛大学医学院附属医院2004年1月至2005年6月由同一组医师完成的NSCLC患者269例,均行标准肺叶切除+区域淋巴结清扫术,全部患者临床资料和随访资料完整,病理标本保留完善,手术前、后均未行放疗和化疗等辅助治疗。应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结法(S-P)检测癌组织标本中VEGF-C表达,以CK19标记检测肺门和纵隔淋巴结微转移的情况,结合患者临床资料、病理结果及随访数据进行统计学分析。结果269例患者的性别(Hc=1.722,P=0.084)、年龄(Hc=0.914,P=0.360)、吸烟情况(Hc=2.440,P=0.295)、病理类型(Hc=5.668,P=0.058)和肿瘤直径(Hc=0.165,P=0.920)间VEGF-C表达差异无统计学意义,不同病理分化程度间VEGF-C表达差异有统计学意义(Hc=29.178,P=0.000);患者CK19检测在性别(χ2=0.000,P=0.999)、年龄(χ2=0.005,P=0.999)、吸烟情况(χ2=2.294,P=0.317)、病理类型(χ2=0.573,P=0.289)、病理分化程度(χ2=2.927,P=0.231)和肿瘤大小(χ2=0.006,P=0.999)间差异无统计学意义;VEGF-C表达强度不同时5年生存率差异有统计学意义(χ2=37.318,P=0.000);CK19阳性和阴性表达5年生存率差异有统计学意义(χ2=39.987,P=0.000);VEGF-C表达与CK19阳性率之间差异有统计学意义(χ2=25.954,P=0.000)。结论 VEGF-C表达、CK19结果与Ⅰ期NSCLC患者术后5年生存率关系密切,VEGF-C、CK19检测有助于判断患者预后,并指导患者手术后辅助治疗,具有较大的临床意义。Objective To evaluate the significance of expression of vascular endothelial growth factor-C （VEGF-C） and cytokeratin 19 （CK19） in patients with stage I non-small cell lung cancer （NSCLC）. Methods A total of 269 patients with NSCLC who underwent standard lobectomy and lymph node dissection by the same surgical team in our hospital from January 2004 to June 2005 were included in this study. All the clinical data and follow-up results were complete, and all the pathological specimens were well kept. No preoperative or postoperative adjuvant therapy such as radiotherapy and chemotherapy was administered to those patients. Expressions of VEGF-C in cancer tissues was detected by immunohistochemical streptavidin-peroxidase （S-P） method, and CK19 was marked to examine micrometastasis in hilar and mediastinal lymph nodes. Clinical outcomes, pathological results and follow-up data were analyzed in combination with VEGF- C and CK19 expression. Results VEGF-C expression was not statistically different between different category in sex （Hc=1.722, P=0.084）, age （Hc=0.914, P=0.360）, smoking （Hc=2.440, P=0.295 ）, pathology type （Hc=5.668, P=0.058 ）or tumor size （Hc=0.165, P=0.920）. VEGF-C expression was statistically different between different groups of pathological differentiation （Hc=29.178, P=0. 000）. CK19 expression was not statistically different between different category in sex （X2=0.000, P=0.999）, age （X2=0.005, P=0.999）, smoking （x2=2.294, P=0.317）, pathology type （X2=0.573, P=0.289 ）, tumor size （X2=0.006, P=0.999）, and pathological differentiation （x2=2.927, P=0.231 ）. Five-year survival rate was statistically different between different grade of VEGF-C expression （x2=37.318, P=0. 000）, and was also statistically different between positive group and negative group of CK19 （x2=39.987, P=-0. 000）. There was statistical difference between different grade of VEGF-C expression and positive rate of CK19 （x2=25.954, P=0. 000）. Conclusion Expression

关 键 词：非小细胞肺癌 血管内皮生长因子C 细胞角蛋白19 

分 类 号：R734.2[医药卫生—肿瘤]