兔关节软骨细胞在几丁糖中培养的生物学特性  

Biological Behavior of Rabbit Articular Chondrocyte in Chitosan Gels

作　　者：郑卫平[1] 张志光[1] 郑有华[1] 杨国平[1] 

机构地区：[1]中山医科大学口腔医学院口腔颌面外科,广东广州510055

出　　处：《中山医科大学学报》2000年第4期277-279,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：香港杨震基金资助课题

摘　　要：:【目的】观察关节软骨细胞在几丁糖凝胶中培养的生物学行为 ,以探讨几丁糖凝胶作为组织工程细胞支架的可行性。【方法】从幼龄兔关节表面削取薄层软骨 ,经酶消化获得大量的软骨细胞。原代培养后 ,按 10 6/mL细胞浓度接种于几丁糖凝胶 ,通过绘制生长曲线 ,组织化学检测 ,透射电镜观察 ,了解软骨细胞在几丁糖中生长特征。【结果】软骨细胞在几丁糖中保持圆形 ,甲苯胺蓝异染及Ⅱ型胶原免疫组化染色呈强阳性 ;细胞内富含高尔基体、粗面型内质网及大量的分泌泡。【结论】软骨细胞在几丁糖中保持圆形生长状态 ,几丁糖能促进软骨细胞分裂、增殖 ,提高其分泌基质的能力。Objective To observe the biological behavior of chondrocyte in chitosan gels in vitro and the possibility of chitosan using as scaffolds in tissue engineering. Methods Chondrocytes isolated by enzymic digestion from the condylar cartilages of the young rabbits were cultured in monolayer system. After primary culture, the cells were seeded in chitosan gels in concentration of 10 6/mL in vitro. Characteristics of chondrocyte growth were observed with growth curve, toluidine blues staining, type Ⅱ collagen immunohistochemistry reaction and also observed by electronic microscope. Result Chondrocytes retained their surrounded morphology, multiplited, and produced matrix in chitosan gels. The results of histochemistry examination were strong postive. Most cells appeared to contain several golgi zones and large areas of rough endoplasmic reticulum, partly filled with dense secretory material. Conclusion Cell culture system, using chitosan gels, provided chondrocytes a suitable environment in which synthesized matrix macromolecules and preserved their surrouned morophology in vitro. Chitosan gels may be a vehicle for injectable tissue engineered cartilage.

关 键 词：关节软骨细胞 几丁糖凝胶 细胞培养 生物学特性 

分 类 号：R782[医药卫生—口腔医学]