IFN-α联合IL-6对CGL患者骨髓细胞生长及生长-凋亡相关基因表达的影响  

Effects of IFN-α combined with IL-6 on cell growth and related genes expression and apoptosis of bone marrow cells from CGL patients

作　　者：陈汉春[1] 汤立军[1] 彭兴华[1] 罗志勇[1] 罗赛群[1] 谭文斌[1] 

机构地区：[1]湖南医科大学分子生物学研究中心,长沙410078

出　　处：《中华血液学杂志》2000年第7期341-344,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金!资助项目 (39570 80 5)

摘　　要：目的　探讨α干扰素 (IFN α)及IFN α联合白细胞介素 6 (IL 6 )对慢性粒细胞白血病(CGL)患者骨髓单个核细胞生长及bcr abl、bcl 2和c myc基因表达的影响。方法　以 2 0 0U mlIFN α或2 0 0U mlIFN α联合 10 0ng mlIL 6液体培养CGL慢性期患者骨髓单个核细胞 ,采用逆转录 聚合酶链反应 (RT PCR)相对定量分析培养 2 4h的骨髓单个核细胞中 β actin、bcr abl、bcl 2和c myc基因表达水平。结果　IFN α明显抑制细胞生长 ,IL 6可在IFN α作用的基础上轻微降低IFN α对细胞生长的抑制程度 ;IFN α或IFN α加IL 6共同作用均抑制bcr abl和bcl 2基因表达 ;IFN α抑制c myc基因表达 ,IL 6则促进c myc基因表达。结论　IFN α及IFN α联合IL 6均抑制bcr abl和bcl 2等抗凋亡基因表达 ,并调节c myc基因表达水平。通过促进细胞凋亡而抑制恶性克隆增长是IFN α或IFN α联合IL 6治疗CGL的可能作用机制。Objective To investigate the effects of interferon α (IFN α) and IFN α combined with interleukin 6 (IL 6) on cell growth and bcr/abl, bcl 2 and c myc genes expression in the bone marrow mononuclear cells (MNC) from chronic granulocytic leukemia (CGL) patients. Methods MNCs were cultured in liquid medium at the presence of IFN α(200?U/ml) or IFN α(200?U/ml) plus IL 6 (100?ng/ml). The viable cells were counted and the expression levels of β actin, bcr/abl, bcl 2 and c myc genes were quantitatively detected by reverse transcriptase polymerase chain reaction (RT PCR). Results The cell growth was markedly inhibited by IFN α, but the extent of the inhibition was slightly decreased when IFN α combined with IL 6. The expression levels of bcr/abl and bcl 2 gene were reduced by IFN α or IFN α plus IL 6. The expression of c myc gene was inhibited by IFN α but promoted by IL 6. Conclusions Both IFN α and IFN α plus IL 6 can inhibit the expression of anti apoptosis genes, and modulate the expression of c myc. It is the possible mechanism of IFN α therapy for CGL in chronic phase.

关 键 词：慢性粒细胞白血病 骨髓 细胞凋亡 IFN-Α IL-6 

分 类 号：R733.7[医药卫生—肿瘤]