Streptomyces griseus ATCC 13273对去氢骆驼蓬碱的转化研究  

Study on Biotransformation of Harmine by Streptomyces griseus ATCC 13273

作　　者：李琳[1] 向文[1] 余伯阳[1] 刘吉华[1] 

机构地区：[1]中国药科大学中药复方研究室,南京211198

出　　处：《药物生物技术》2012年第4期299-303,共5页Pharmaceutical Biotechnology

基　　金：江苏高校优势学科建设工程资助项目(PAPD)资助

摘　　要：利用微生物转化的方法,选取灰色链霉菌(Streptomyces griseus ATCC 13273)为转化菌株,对去氢骆驼蓬碱的转化进行研究。该菌株可以将去氢骆驼蓬碱转化为两种转化产物(记为H1和H2),通过MS、1H-NMR和13C-NMR分析,转化产物分别鉴定为哈尔酚和N-羟基去氢骆驼蓬碱。并对转化条件进行初步优化,优化后的转化条件为:初始pH 8.0,两步活化法以4%的接种量转接,48 h后加入底物(以N,N-二甲基甲酰胺溶解),发酵液中去氢骆驼蓬碱终浓度为0.25 mmoL/L,发酵时间为5 d。转化条件优化后H1和H2的产率分别达到8.70%和12.76%;该研究丰富了S.griseus ATCC 13273的作用底物范围,为哈尔酚和N-羟基去氢骆驼蓬碱的合成提供了一条新途径。Biotransformation studies of harmine by S. griseus ATCC 13273 were conducted. S. griseus ATCC 13273 catalyzed the mi- crobial transformation process of harmine into two metabolites （ HI and H2）. The two metabolites were isolated and identified as har- mol and N-hydroxyl-harmine by MS, 1H-NMR and 13 C-NMR analysis. The optimization of harmine biotransformation conditions was carried out and the optimal conditions were determined as follows：pH value 8.0, substrate concentration 0.25mmoL/L,fermentation time 5 d, addition time 48 h,inoculums concentration 4% , and addition mode dissolved in DMF. Under this optimized condition, the yield of harmol and N-hydroxyl-harmine was 8.70% and 12.76% ,respectively. This study enriches the range of substrate S. gr/seus ATCC 13273 catalysis providing a new approach to synthesize harmol and N-hydroxyl-harmine.

关 键 词：灰色链霉菌 去氢骆驼蓬碱 哈尔酚 N-羟基去氢骆驼蓬碱 微生物转化 产率 

分 类 号：TQ92[轻工技术与工程—发酵工程]