Determination of Sulfadiazine Based on Its Derivatization with Fluorescamine by Self-Ordered Ring Fluorescence Microscopic Imaging Technique  

作　　者：YANG Le LIU Ying 

机构地区：[1]College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China [2]Yinchuan No. 2 High School, Ningxia 750004, China

出　　处：《光谱学与光谱分析》2012年第9期2458-2463,共6页Spectroscopy and Spectral Analysis

基　　金：The National Nature Science Foundation of China(Grant Nos.20767004,21177163);111 Project B08044;"985 Project"of Minzu University of China(MUC 98507-08);"211 Project"of Minzu University of China(MUC 02121103)

摘　　要：A self-ordered ring(SOR) fluorescence microscopic imaging technique has been developed for the determination of trace amounts of sulfadiazine based on its derivatization with fluorescamine.In the presence of HAc-NaAc buffer solution(pH 3.12) and polyvinyl alcohol-124(PVA-124),the droplet containing fluorescamine derivatized sulfadiazine can form a SOR on the solid support after solvent evaporation with the diameter of 1.86mm and its ring belt width of 54.9 μ m.The quantitative analysis of sulfadiazine is achieved with the linear range of 7.8×10-14 ～1.8×10-12 mol·ring-1(3.9×10-7 ～9.0×10-6 mol·L-1) and detection limit of 7.8×10-15 mol·ring-1(3.9×10-8 mol·L-1) when 0.2 μ L droplet was spotted.The technique has been satisfactorily applied to the determination of sulfadiazine in the tablet,synthetic sample and residues in six different milk samples with the recoveries of 91.0%～105.8%,respectively,and RSDs less than 4.4%.A self-ordered ring（SOR） fluorescence microscopic imaging technique has been developed for the determination of trace amounts of sulfadiazine based on its derivatization with fluorescamine.In the presence of HAc-NaAc buffer solution（pH 3.12） and polyvinyl alcohol-124（PVA-124）,the droplet containing fluorescamine derivatized sulfadiazine can form a SOR on the solid support after solvent evaporation with the diameter of 1.86mm and its ring belt width of 54.9 μ m.The quantitative analysis of sulfadiazine is achieved with the linear range of 7.8×10-14 ～1.8×10-12 mol·ring-1（3.9×10-7 ～9.0×10-6 mol·L-1） and detection limit of 7.8×10-15 mol·ring-1（3.9×10-8 mol·L-1） when 0.2 μ L droplet was spotted.The technique has been satisfactorily applied to the determination of sulfadiazine in the tablet,synthetic sample and residues in six different milk samples with the recoveries of 91.0%～105.8%,respectively,and RSDs less than 4.4%.

关 键 词：SOR SULFADIAZINE FLUORESCAMINE DERIVATIZATION 

分 类 号：O657.3[理学—分析化学]