镜泊湖水体水溶性有机物荧光特性研究  

Fluorescence Spectroscopic Characteristics of Dissolved Organic Matters(DOM) from Jingpo Lake Water

作　　者：王曼霖[1] 席北斗[2] 许其功[2] 赵越[1] 魏自民[1] 张斌[1] 白雪[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]中国环境科学研究院水环境系统工程研究室,北京100012

出　　处：《光谱学与光谱分析》2012年第9期2477-2481,共5页Spectroscopy and Spectral Analysis

基　　金：水体污染控制与治理科技重大专项课题项目(2009ZX07106-001)资助

摘　　要：通过黑龙江省区域镜泊湖水体6个点位样品采集(样品号J1-J6),利用荧光检测技术,结合三维荧光光谱区域积分(FIR),研究了水溶性有机物(DOM)的荧光特性。传统荧光光谱显示J4和J5DOM分子缩合度较高;三维荧光光谱显示J6点位DOM中类蛋白特征峰最为显著;对所有点位DOM的三维荧光光谱5个区域积分(AⅠ,AⅡ,AⅣ:类蛋白区域;AⅢ:类富里酸区域:AⅤ:类胡敏酸区域)显示:所有点位DOM区域积分中AⅤ区域占有比例最大,并且以J4和J5点位最高,J6点位最低。通过对腐殖酸区域(AⅢ和AⅤ积分比例之和)与类蛋白区域(AⅠ,AⅡ,AⅣ积分比例之和)积分比值表明,J4(4.94)和J5(5.18)点位比值相近;J1(3.52)和J2(3.66)点位比值接近;最小值为J6点位(2.11)。综合以上分析证实,镜泊湖水体各点位DOM腐殖化程度为:J4,J5>J1,J2>J3>J6点位。Six samples（sample J1—J6） from Jingpo Lake in Heilongjiang province were analyzed by fluorescence and fluorescence excitation-emission matrix regional integration（FIR） to determine the different characteristics of dissolved organic matters（DOM）.The results with the traditional method just analyzing the excitation,emission and synchronous fluorescence spectra indicated that DOM molecular condensation degree was highest at sample J4 and sample J5,however,the study with the three-dimensional-excitation emission matrix spectra（3EEMs） method showed that the content of protein-like material was higher in sample J6 than others.In the second method,3EEMs was divided into five regions,among which Region Ⅰ,Region Ⅱ,and Region Ⅳ were related to protein-like material,Region Ⅲ was related to fulvic acid-like material,Region Ⅴ was related to humic acid-like organics,and then these regions were integrated named as AⅠ,AⅡ,AⅢ,AⅣ and AⅤ.The integration results showed that the volume of AⅤ occupied the largest proportion of the DOM region integration from all samples,and it exhibited the most prominent both in sample J4 and sample J5,while it’s opposite in sample J6.Integral ratio,which means humic acid-like region（AⅢ,AⅤ） divided by protein-like region（AⅠ,AⅡ,AⅣ）,showed that the value of J4（4.94） was close to J5（5.18）,J1（3.52） was close to J2（3.66）,and the minimum value appeared in J6（2.11）.From the above analysis,the DOM humification degree could be confirmed as follows： J4,J5〉 J1,J2〉 J3〉 J6.

关 键 词：镜泊湖 水溶性有机物 荧光光谱 区域积分 

分 类 号：X705[环境科学与工程—环境工程]