microRNA-34a与非小细胞肺癌的关系及其可能调节机制  

The relationship between microRNA-34a and non small-cell lung cancer and its possible regulation mechanism

作　　者：曾婷[1] 郝丽[1] 谢逸欣[1] 马丽[1] 舒文杰[2] 

机构地区：[1]广州军区疾病预防控制中心,广东广州510507 [2]解放军军事医学科学院,北京100080

出　　处：《中国医药导报》2012年第23期5-7,共3页China Medical Herald

基　　金：国家自然科学基金青年科学基金项目(30700139)

摘　　要：目的探讨miRNA-34a在非小细胞肺癌发生中的作用及其可能机制。方法选取56例经病理诊断确诊为非小细胞肺癌患者的癌组织及癌旁组织,RT-PCR法测定miRNA-34a及c-myc mRNA的表达水平,免疫组织化学法检测癌组织及癌旁组织的P53蛋白及c-myc蛋白的表达水平。结果癌组织的miRNA-34a的表达水平低于癌旁组织,c-myc mRNA表达及c-myc、P53蛋白表达阳性率高于癌旁组织(P<0.05)。miRNA-34a与c-myc mRNA表达水平呈负相关关系(P<0.05)。结论 miRNA-34a对非小细胞肺癌的发生具有一定的调节作用,可能是非小细胞肺癌发生的重要环节,miRNA-34a主要是通过与c-myc的结合而发挥其调节作用。Objective To explore the effect of miRNA-34a on the occurrence of small cell lung cancer （SCLC） and its pos- sible mechanism. Methods Lung cancer tissues and its corresponding non cancer tissues in 56 cases of patients who diag- nosed as SCLC by pathology were collected for detecting its miRNA and c-myc mRNA expression by RT-PCR. Immuno- histochemistry method was used for detecting P53 and c-myc protein expression in cancer tissue and its corresponding non cancer tissues. Results Compared with corresponding non-cancer tissues, the expression of miRNA-34a in lung cancer tissue was lower, however the expression of e-myc mRNA, c--myc protein and P53 protein were higher （P 〈 0.05）. There was a neg- ative correlation between miRNA-34a and c-myc mRNA expression level （P 〈 0.05）. Conclusion miRNA-34a plays a reg- ulating role on the occurrence of SCLC, and it may be an important factor on SCLC occurrence by combination with c-myc.

关 键 词：miRNA-34a C-MYC P53 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]