Bcl-2 RNA有效靶点的筛选及其对白血病细胞HL-60的促凋亡作用  

Apoptosis Induction of the Mostly Efficient Bcl-2 siRNA to Human Leukemia-60 Cells

作　　者：燕春艳[1] 苏海英[2] 魏红[1] 宋希元[1] 杨庆媛[1] 

机构地区：[1]济宁医学院病理教研室,山东济宁272013 [2]济宁市第一人民医院妇产科,山东济宁272111

出　　处：《中国医药指南》2012年第22期37-39,共3页Guide of China Medicine

摘　　要：目的筛选有效的Bcl-2 siRNA并观察其对白血病细胞HL-60的生长抑制作用。方法用体外转录合成法合成双链Bcl-2 siRNA,将其转染入HL-60细胞;用流式细胞术检测Bcl-2蛋白表达,MTT法、流式细胞术及吉姆萨(Giemsa)染色法观察细胞增殖及凋亡情况。结果有效Bcl-2 siRNA有抑制HL-60细胞生长及促进细胞凋亡作用。结论有效Bcl-2 siRNA能特异性促进HL-60细胞凋亡,抑制其生长。Objective To study the Effect of Bcl-2 siRNA on apoptosis of human leukemia-60 Cells.Methods Bcl-2 siRNA was synthesized in vitro transcription with silencer siRNA construction kit.The synthesized siRNA was transfected into HL-60 cells with Amine siPORT transfection.We used MTT,Giemsa staining and flow cytometer to value cell proliferation and apoptosis.Results Bcl-2 siRNA could partially inhibit the growth of HL-60 cells.After incubated with Bcl-2 siRNA1 for 48 hours,HL-60 cells exhibited morphologic characteristic of apoptosis including chromatin condensation,crescents formation and nuclear fragmentation.Conclusions Effective Bcl-2 siRNA can induce apoptosis and inhibit cell-proliferation.

关 键 词：RNA干扰 小的双链RNA HL60 凋亡 

分 类 号：R73[医药卫生—肿瘤]