低频超声对卵巢癌细胞的生物学效应  

Bioeffects of low-frequency ultrasound on human ovarian carcinoma cell line 3AO

作　　者：于廷和[1] 蔡汉钟[1] 王智彪[1] 江森[2] 

机构地区：[1]重庆医科大学医学超声工程研究所,400016 [2]山东医科大学附属医院妇产科

出　　处：《中华物理医学与康复杂志》2000年第4期237-239,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金："九五"国家科技重点攻关资助项目!(96 90 5 0 2 0 1 )

摘　　要：目的　研究低频超声对体外培养的人卵巢癌细胞的急、慢性生物学效应。方法　人卵巢癌细胞 3AO受不同剂量、频率为 0 .2 4MHz超声波辐照 ,根据剂量 -效应曲线确定两种不致细胞急性损伤的声波剂量 ,观察受此两种剂量超声处理后细胞的增殖能力和克隆形成力的变化。结果　 3 .92W /cm2 × 10s和 5 .12W /cm2 × 5s未致细胞急性死亡。受 3 .92W /cm2 × 10s、5 .12W /cm2 × 5s辐照和对照组细胞倍增时间分别为 45 .2h、3 4 .4h和 2 8.6h ,克隆形成率分别为 2 1.3 %、5 0 .3 %和 46.0 % ,受 3 .92W /cm2 × 10s辐照细胞增殖和克隆形成均受到抑制 (P值均 <0 .0 1)。结论　 3AO对超声的敏感性较高 ,超声的迟发效应更决定于辐照持续时间。Objective To determine acute and delayed effects of low frequency ultrasound on human ovarian carcinoma cell line 3AO in vitro.Methods Cells were irradiated by ultrasound with dosage series and a frequency of 0.24 MHz. The dosages that did not lead to acute cell death were determined and their effects on ability of proliferation and colony forming were inspected. Results 3.92 W/cm 2×10 s and 5.12 W/cm 2×5 s did not lead to cell killing immediately. The doubling times were 45.2 h, 34.4 h and 28.6 h; and the colony forming rates were 21.3 %, 50.3 % and 46.0 % in cells exposure to 3.92 W/cm 2×10 s, 5.12 W/cm 2×5 s and control respectively. Cell proliferation and colony forming were inhibited in cells irradiated by 3.92 W/cm 2×10 s(P< 0.01 ,P< 0.01 ). Conclusion 3AO was sensitive to ultrasound. The delayed effects depended more on duration of irradiation than on acoustic intensity.

关 键 词：卵巢肿瘤 生物学效应 低频超声 

分 类 号：R737.31[医药卫生—肿瘤]