重组人骨形成蛋白-2、胶原、珊瑚复合人工骨异位诱导成骨的实验研究  

Experimental study on the bone inductivity of rhBMP_2 collagen and coral complex

作　　者：尉文华[1] 毛天球[2] 陈富林[2] 王贺忠 张忠友 

机构地区：[1]张家口市二五一医院口腔科,075000 [2]第四军医大学口腔医学院颌面外科

出　　处：《现代口腔医学杂志》2000年第4期231-232,共2页Journal of Modern Stomatology

摘　　要：目的　采用珊瑚作为载体 ,胶原作为缓释系统 ,制备出重组人骨形成蛋白 - 2 (rhBMP - 2 )胶原 /珊瑚复合人工骨 ,并评价其骨诱导活性。方法　rhBMP - 2、胶原和珊瑚以一定的方式复合后 ,植入小鼠股部肌袋内 ,以单纯珊瑚植入作对照 ,术后不同时间取材 ,通过组织学方法检测骨诱导活性。结果　复合人工骨植入 1周诱导软骨形成 ,2周形成编织骨 ,4周形成含骨髓的板层骨 ,同时珊瑚被部分降解吸收。结论　胶原、珊瑚是rhBMP - 2较理想的载体 ,有缓释BMP的作用 ,该复合骨具有良好的异位诱骨活性 。Objective To observe the bone inductive activity of rhBMP-2/collagen/coral complex.Methods rhBMP-2/collagen/coral complex was implanted into mouse muscle pouch.The samples were observed by histological examination at 1.2.4.weeks post operation.Results 1 week after implantation,neo-cartilage could be seen around the coral , 2 weeks after implantation,woven bone formed and lamellar bone containing bone marrow formed 4w after implantation,and coral degraded partially.Conclusion Coral is a good carrier of rhBMP-2 and collagen in the complex acts as the delivery system for rhBMP-2.This complex is useful for bone defect repair because it has both bone conductive and bone inductive activity.

关 键 词：RHBMP-2 胶原 珊瑚 复合人工骨 骨诱导 

分 类 号：R318.08[医药卫生—生物医学工程]