ICAM-1/LFA-1分子表达与大鼠肝脏缺血再灌注损伤的关系  

The relationship between the expression of intercellular molecule-l/lymphocyte func-tion-associated antigen-l and rat liver ischemia-reperfusion injury

作　　者：陈剑琳[1] 何晓顺[2] 傅宇阳[2] 

机构地区：[1]中山医科大学病理学教研室,510089 [2]中山医科大学附属第一医院器官移植中心,510080

出　　处：《广州医药》2000年第4期3-4,共2页Guangzhou Medical Journal

摘　　要：目的：探讨 ＩＣＡＭ－１／ＬＦＡ－１分子表达与大鼠肝脏缺血再灌注损伤的关系。方法：将４０只实验大鼠分为两组，分别阻断左叶及中叶肝脏的门静脉分支 ３０ ｍｉｎ和 ６０ｍｉｎ。所有大鼠在去阻断血流４８ ｈ后杀死，取肝组织做冰冻及石蜡切片，进行免疫组化分析及组织学观察。结果：在阻断血流 ３０ ｍｉｎ的实验组中，未见明显的肝组织学损伤，肝组织内 ＩＣＡＭ－１及ＬＦＡ－１仅有微弱表达，而在阻断血流 ６０ ｍｉｎ的实验组中，肝组织损伤严重，肝组织内ＩＣＡＭ－１和ＬＦＡ－１的表达明显增强。结论：肝组织内ＩＣＡＭ－１和ＬＦＡ－１的表达水平与肝脏缺血再灌注损伤的程度密切相关。Objective: To investigate the relationship between the expression of intercellular adhesionmolecule-l/lymPhocyte function-associated antigen-l and rat liver ischemia-reperfusion injury. Meth-ods: Forty adult Wistar rats were randomized into 2 groups. The hepatic hilar Vessels distributing to the leftand median lobes were clamped for 30 min and 60 min respectively. All rats were killed 48 h after declamp-ing and specimens of liver tissue were obtained. Frozen and paraffin sections were analyzed through immuno-histochemistry and histology. Results: In the 30 min group, no evident histological liver in jnjury was found.The expression of ICAM-l and LFA-l on liver tissue was delicate. However, after 60 min warm ischem-a, reperfusion induced severe liver damages. The eXpression of ICAM -l and LFA-l was also notable.Conclusion: The expression level of ICAM-l and LFA-l is closely associated with the degree of liver is-chemia-reperfusion injury.

关 键 词：肝脏缺血 再灌注损伤 ICAM-1 LFA-1 

分 类 号：R363[医药卫生—病理学]