转化生长因子-β1基因启动子多态性与肝癌关系的研究  

Association of the TGF-β1 gene promoter polymorphisms with hepatocellular carcinoma

作　　者：韦忠恒[1] 陆建勋[1] 浦涧[1] 王子锡[2] 牙韩年[2] 唐任光[3] 龙显科[2] 

机构地区：[1]右江民族医学院附属医院肝胆外科,广西百色533000 [2]右江民族医学院附属河池市凤山县人民医院普外科 [3]右江民族医学院附属医院检验中心,广西百色533000

出　　处：《肿瘤研究与临床》2012年第7期447-450,共4页Cancer Research and Clinic

基　　金：广西卫生厅资助课题（Z2011125）

摘　　要：目的研究转化生长因子β1（TGF-β1）基因启动子多态性各等位基因及基因型在肝癌（HCC）患者中的分布频率，初步分析其基因型及血清水平与HCC的相关性。方法采用限制性片段长度多态性聚合酶链反应（PCR-RFLP）技术，检测102例HCC患者和110例健康对照TGF—β1的基因多态性，包括TGF-13，基因启动子-800G／A、-509C／T位点，采用酶联免疫吸附试验（ELISA）检测血清TGF—β1水平。结果HCC患者TGF-β1水平显著高于对照组[（51．06±9．74）txg／L，（22．12±8．67）μg／L，t=22．884，P〈0．01]，TGF—β1基因-800G／A位点多态性在HCC组和健康对照组中的分布差异无统计学意义（P〉0．05），而TGF-β1基因-509C／T多态性各等位基因及基因型频率在两组人群中的分布差异有统计学意义（P〈0．05）；等位基因频率的相对风险分析发现，T等位基因携带者患HCC的风险是C等位基因的1．822倍（OR=1．822，95％凹：1．238—2．682，t=22．884，P〈0．01），携带T等位基因的HCC患者血清TGF-β1水平高于不携带者[（53．52±10．07）μg／L，（43．57±9．89）μg／L，t=3．898，P〈0．01]。结论TGF-β1基因-509C／T多态性与HCC之间存在相关关系；携带T等位基因的个体可能通过促进TGF—B。的高度表达而增加HCC的发病风险。Objectives To study the relationship between the allele frequencies and genotype distribution of transforming growth factor-beta 1 （TGF-β1） gene promoter polymorphisms in Chinese patients with heptocellular carcinoma（HCC）, and to analyze the association of the serum levels and genotype of TGF-β1 with HCC. Methods The polymorphisms of TGF-β1 gene, including polymorphisms of TGF-β1 gene -800G/A, -509C/T, were analyzed by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） methods in 102 patients with HCC and 110 healthy controls, and the serum level of TGF-β1 was determined by enzyme-linked immunosorbent assay （ELISA）. Results The HCC group showed significantly higher serum levels of TGF-β1 than control group [（51.06 ± 9.74）μg/L ,（22.12 ± 8.67 ）μg/L ,t=22.884, P〈0.01], The distributions of TGF-β1 gene -800G/A polymorphisms were not different significantly between HCC group and control group, but TGF-β1 -509C/T gene polymorphism was significantly different. The relative risk suffered from HCC of C allele was 1.822 times of the T allele （OR=1.822,95 %CI： 1.238-2.682, t=22.884,P〈0.01）, the serum level of TGF-β1 T allele carriers was significantly higher than that of no carriers [（53.52±10.07） μg/L, （43.57±9.89）μg/L, t=3.898, P〈0.01]. Conclusion TGF-f51 gene -509C/T polymorphism is associated with HCC, and T allele may be a risk factor for HCC, in which the TGF-131 T allele carriers may have increased risk by enhancing the TGF-15~ expression in the pathogenesis of HCC.

关 键 词：肝肿瘤 转化生长因子Β1 基因 多态性 

分 类 号：R735.7[医药卫生—肿瘤]