胃癌、癌旁组织中抑癌基因和凋亡调节基因表达及其内在关系  

Expression and mutual relationship of tumor suppression gene and apoptosis regulating gene in gastric carcinoma and paracancerous tissues

作　　者：郜恒骏[1] 白剑峰[1] 彭延申 孙谷[1] 赵翰林[1] 吕秀珍[1] 赵志泉[1] 萧树东[2] 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院210029 [2]上海市消化疾病研究所

出　　处：《中华消化杂志》2000年第3期178-181,共4页Chinese Journal of Digestion

基　　金：江苏省教委自然科学基金!( 12 5FA960 8)

摘　　要：目的　研究胃癌、癌旁组织中抑癌基因 p5 3、p16和关键性凋亡调节基因bcl 2蛋白的表达及其内在的关系 ,进一步探讨胃癌发病可能的分子机制。方法　术中取 30例胃癌患者的癌组织 ,癌旁组织各 2块 ,石蜡包埋 ,切片分别作HE染色病理检查及免疫组织化学检查 p5 3、p16、bcl 2基因蛋白表达。结果　p5 3、p16和bcl 2表达与胃癌分期、分化程度及有无淋巴结转移均无显著关系 (P >0 .0 5 ) ,但bcl 2与胃癌类型显著相关 (P <0 .0 5 ) ;p5 3基因在胃癌中阳性表达率显著高于中、重度异型增生 (P <0 .0 5 ) ;p16在慢性胃炎中阳性表达率显著高于胃癌 (P <0 .0 1)、不完全型肠化和异型增生 (P <0 .0 5 ) ;bcl 2在异型增生组织中的阳性表达率与胃癌、不完全型肠化相比差异均无显著性 (P >0 .0 5 ) ,异型增生、胃癌组织中bcl 2的阳性表达率均显著高于慢性胃炎 (P <0 .0 0 1) ;p5 3与p16、p16与bcl 2表达之间均无显著相关性 (P >0 .0 5 ) ,而 p5 3与bcl 2表达之间存在显著相关性 (P <0 .0 5 )。结论　p5 3基因过度表达是胃癌发展过程中较晚期事件 ,主要与胃癌进展有关 ;在胃癌发生的早期即存在较明显的p16基因表达低下与bcl 2基因过度表达且后者与肠型胃癌发生的关系似乎更为密切。在胃癌发生发展过程中p5 3、p16及bclObjective To investigate the expression and mutual relationship of tumor suppression gene and apoptosis regulating gene in gastric carcinoma and paracancerous tissues. Methods 30 cases of gastric carcinoma patients underwent surgical resection. Samples were taken from tumor site and paracancerous tissues. ABC immunohistochemical staining was used to detect the expression of p53、p16、bcl 2 proteins. Results No correlation between p53, p16, or bcl 2 expression and grade of pathologic stage, grade of differentiation, or lymph node status was observed ( P >0.05), there was significant correlation between bcl 2 and type of gastric carcinoma ( P <0.05). The positive rate of p53 gene expression in gastric carcinoma was significantly higher than that in moderate and severe dysplasia ( P <0.05). The positive rate of p16 gene expression in chronic gastritis was significantly higher than that in gastric cancer ( P <0.01), in incomplete intestinal metaplasia or dysplasia ( P <0.05). There was no significant difference for the positive rate of bcl 2 gene expre ssion between dysplasia and gastric cancer or incomplete intestinal metaplasia ( P >0.05), The positive rate of bcl 2 expression in dysplasia or gastric cancer was significantly higher than that in chronic gastritis (P< 0.001). There was no significant correlation between p53 and p16, nor between p16 and bcl 2 ( P >0.05), whereas there was significant correlation between p53 and bcl 2 ( P <0.05). Conclusions The p53 gene overexpression is relatively late event in stomach tumorigenesis and mainly relate to the progression. p16 or bcl 2 protein expression alteration might play a role in the early development/promotion of gastric carcinoma but not in tumor progression and aberrant bcl 2 protein expression appears to be preferentially associated with the intestinal type tumors. p53, p16 and bcl 2 play both independant and synergetic role in the course and development of gastric cancer.

关 键 词：胃肿瘤 癌前病变 P53基因 P16基因 BCL-2基因 

分 类 号：R735.2[医药卫生—肿瘤]