响应面分析法优化藤茶中二氢杨梅素的提取工艺  

Optimization of Extraction Technology for Dihydromyricetin from Ampelopsis grossedentata by Response Surface Analysis Methodology

作　　者：谢郁峰[1] 陈卓瀚[1] 吕华冲[1] 

机构地区：[1]广东药学院药科学院药物化学系,广州510006

出　　处：《中国实验方剂学杂志》2012年第17期46-49,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选藤茶中二氢杨梅素的提取工艺。方法:以二氢杨梅素提取率为考察指标,在单因素试验基础上,选取乙醇体积分数、料液比、提取时间为自变量,二氢杨梅素提取率为响应值,采用三因素三水平的响应面分析法优选二氢杨梅素的提取工艺条件。结果:最佳提取工艺条件为17倍量81%乙醇回流提取95 min。在此条件下,二氢杨梅素提取率可达9.58%,与理论值9.72%仅相差0.14%。结论:采用响应面法优选的二氢杨梅素提取工艺是可行的。Objective： To optimize extraction process of dihydromyricetin from Ampelopsis grossedentata by response surface methodology. Method： With extraction ratio of dihydromyricetin as index, based on single factor test, the concentration of ethanol, liquid-solid ratio and extraction time were selected as independent variables and extraction rate of dihydromyricetin as response value, response surface methodology with three-factors and three-levels was carried out for optimizing extraction process of dihydromyricetin from A. grossedentata. Result： Optimum extraction conditions of dihydromyricetin were as followed： refluxing extracted 95 min with 17 times the amount of 81% ethanol. Under these conditions, extraction ratio of dihydromyricetin was 9.58%, relative error in comparison with the predicted one was 0. 14%. Conclusion： This optimized technology was reliable, it showed that was feasible for optimizing extraction technology of dihydromyricetin by response surface methodology.

关 键 词：响应面 藤茶 二氢杨梅素 提取工艺 

分 类 号：R283.6[医药卫生—中药学]